Anti-Human BRD2 Polyclonal Antibody (HB192014)

Name: Anti-Human BRD2 Polyclonal Antibody
Brand: abinScience
SKU: HB192014
Price: 96 USD
Availability: InStock

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Aperçu
Images
Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

HB192014

Reactivité des espèces

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human BRD2 (Arg74-Gln182).

Cible

Really interesting new gene 3 protein, KIAA9001, O27.1.1, BRD2, RING3, Bromodomain-containing protein 2

Purification

Purified by antigen affinity column.

Numéro d'accès

P25440

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Bromodomain-containing protein 2 (BRD2) is a ~88 kDa protein. Chromatin reader protein that specifically recognizes and binds histone H4 acetylated at 'Lys-5' and 'Lys-12' (H4K5ac and H4K12ac, respectively), thereby controlling gene expression and remodeling chromatin structures. Recruits transcription factors and coactivators to target gene sites, and activates RNA polymerase II machinery for transcriptional elongation. Plays a key role in genome compartmentalization via its association with CTCF and cohesin: recruited to chromatin by CTCF and promotes formation of topologically associating domains (TADs) via its ability to bind acetylated histones, contributing to CTCF boundary formation and enhancer insulation. Also recognizes and binds acetylated non-histone proteins, such as STAT3. Involved in inflammatory response by regulating differentiation of naive CD4(+) T-cells into T-helper Th17: recognizes and binds STAT3 acetylated at 'Lys-87', promoting STAT3 recruitment to chromatin.

1. Nakamura, Y. et al. (2007) The Journal of biological chemistry 282, 4193-201. PMID: 17148447

2. Huang, H. et al. (2007) BMC structural biology 7, 57. PMID: 17848202

3. LeRoy, G. et al. (2008) Molecular cell 30, 51-60. PMID: 18406326

4. Umehara, T. et al. (2010) The Journal of biological chemistry 285, 7610-8. PMID: 20048151

5. Umehara, T. et al. (2010) FEBS letters 584, 3901-8. PMID: 20709061

6. Filippakopoulos, P. et al. (2010) Nature 468, 1067-73. PMID: 20871596

7. Cheung, KL. et al. (2017) Molecular cell 65, 1068-1080.e5. PMID: 28262505

8. Xie, L. et al. (2022) Nature genetics 54, 481-491. PMID: 35410381

Note

For research use only.

Images

Western blot

Lane 1: C2C12 cell lysate

Reférences

Fiche technique

Anti-Human BRD2 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

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