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Anti-Human CBX5 Polyclonal Antibody
Anti-Human CBX5 Polyclonal Antibody
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Спецификация:
  • 20ug
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  • 100ug
Количество:
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  • Даташит

  • SDS

Обзор
Каталожный номерHW777014
Реактивность видовHuman, Mouse
ПримененияELISA, IHC, WB
Хозяинский видRabbit
КлоональностьPolyclonal
ИзотипIgG
Иммуноген E. coli - derived recombinant Human CBX5 (Gly2-Lys80).
Цель Heterochromatin protein 1 homolog alpha, Chromobox protein homolog 5, HP1A, Antigen p25, HP1 alpha, CBX5
Очистка Purified by antigen affinity column.
Номер доступа P45973
Форма Liquid
Буфер хранения 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта
Применение Разбавление
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Фон

Chromobox protein homolog 5 (CBX5) is a ~22 kDa protein. Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). Also recognizes and binds histone H1.4 methylated at 'Lys-26' (H1.4K26me). Excluded from chromatin when histone H1.4 is Simultaneously methylated at Lys-26 (H1.4K26me) and phosphorylated at Ser-27 (H1.4S27Ph). May contribute to the association of heterochromatin with the inner nuclear membrane by interactions with the lamin-B receptor (LBR).

1. Zhou, L. et al. (2025) Science (New York, N.Y.) 389, 309-316. PMID: 40440427
2. Dawson, MA. et al. (2009) Nature 461, 819-22. PMID: 19783980
3. Daujat, S. et al. (2005) The Journal of biological chemistry 280, 38090-5. PMID: 16127177
4. Kiyomitsu, T. et al. (2010) The Journal of cell biology 188, 791-807. PMID: 20231385
Примечание For research use only.
Изображения
  • Anti-Human CBX5 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-CBX5 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: MCF-7 cell lysate
    Lane 2: K562 cell lysate
    Lane 3: PC-12 cell lysate
    Lane 4: NIH3T3 cell lysate
    Lane 5: A549 cell lysate

Ссылки
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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