Anti-Human CCL2/MCP-1 Recombinant Antibody (11k2) (HY280107)

Name: Anti-Human CCL2/MCP-1 Recombinant Antibody (11k2)
Brand: abinScience
SKU: HY280107
Price: 191 USD
Availability: InStock

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Каталожный номер	HY280107
Реактивность видов	Human
Применения	Blocking, ELISA, FCM
Хозяинский вид	Mouse
Клоональность	Monoclonal
Изотип	IgG2a, kappa
ID клона	11k2
Цель	HC11, MCP-1, MCP1, Monocyte chemotactic protein 1, Monocyte chemotactic and activating factor, Small-inducible cytokine A2, SCYA2, MCAF, Monocyte secretory protein JE, C-C motif chemokine 2, Monocyte chemoattractant protein 1, CCL2
Уровень эндотоксинов	Please contact with the lab for this information.
Чистота	>95% as determined by SDS-PAGE.
Очистка	Protein A/G purified from cell culture supernatant.
Номер доступа	P13500
Форма	Liquid
Буфер хранения	0.01M PBS, pH 7.4, 0.09% Sodium azide. Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Устойчивость и хранение	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Фон	C-C motif chemokine 2 (CCL2/MCP-1) is a ~11 kDa protein. Acts as a ligand for C-C chemokine receptor CCR2. Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions. Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. 1. Hemmerich, S. et al. (1999) Biochemistry 38, 13013-25. PMID: 10529171 2. Jarnagin, K. et al. (1999) Biochemistry 38, 16167-77. PMID: 10587439 3. Paavola, CD. et al. (1998) The Journal of biological chemistry 273, 33157-65. PMID: 9837883 4. Zhang, YJ. et al. (1994) The Journal of biological chemistry 269, 15918-24. PMID: 8195247 5. Weber, M. et al. (1996) The Journal of experimental medicine 183, 681-5. PMID: 8627182 6. Chakravarty, L. et al. (1998) The Journal of biological chemistry 273, 29641-7. PMID: 9792674 7. Li, YS. et al. (1993) Molecular and cellular biochemistry 126, 61-8. PMID: 8107690
Примечание	For research use only.

Изображения

Bioactivity

Detects CCL2/MCP-1 in indirect ELISAs.
Flow Cytometry

Flow-cytometry using anti-human CCL2 antibody.A549 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CCL2 antibody monoclonal antibody (Catalog # HY280107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human CCL2 antibody.LPS treated THP-1 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CCL2 antibody monoclonal antibody (Catalog # HY280107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.