

| Catalog No. | HY280117 |
|---|---|
| Species reactivity | Human |
| Applications | ELISA, FCM |
| Host species | Mouse |
| Isotype | IgG2a, kappa |
| Clone ID | 11k2 |
| Conjugation | FITC |
| Clonality | Monoclonal |
| Target | HC11, MCP-1, MCP1, Monocyte chemotactic protein 1, Monocyte chemotactic and activating factor, Small-inducible cytokine A2, SCYA2, MCAF, Monocyte secretory protein JE, C-C motif chemokine 2, Monocyte chemoattractant protein 1, CCL2 |
| Endotoxin level | Please contact with the lab for this information. |
| Accession | P13500 |
| Form | Liquid |
| Storage buffer | 0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
| Stability and Storage | Store at 4°C for 12 months. Protect from light. Do not freeze. |
| Background | C-C motif chemokine 2 (CCL2/MCP-1) is a ~11 kDa protein. Acts as a ligand for C-C chemokine receptor CCR2. Signals through binding and activation of CCR2 and induces a strong chemotactic response and mobilization of intracellular calcium ions. Exhibits a chemotactic activity for monocytes and basophils but not neutrophils or eosinophils. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. 1. Hemmerich, S. et al. (1999) Biochemistry 38, 13013-25. PMID: 10529171 2. Jarnagin, K. et al. (1999) Biochemistry 38, 16167-77. PMID: 10587439 3. Paavola, CD. et al. (1998) The Journal of biological chemistry 273, 33157-65. PMID: 9837883 4. Zhang, YJ. et al. (1994) The Journal of biological chemistry 269, 15918-24. PMID: 8195247 5. Weber, M. et al. (1996) The Journal of experimental medicine 183, 681-5. PMID: 8627182 6. Chakravarty, L. et al. (1998) The Journal of biological chemistry 273, 29641-7. PMID: 9792674 7. Li, YS. et al. (1993) Molecular and cellular biochemistry 126, 61-8. PMID: 8107690 |
| Note | For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only. |




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