Anti-Human PDGF R alpha Recombinant Antibody (SAA0057) (HB927207)

Name: Anti-Human PDGF R alpha Recombinant Antibody (SAA0057)
Brand: abinScience
SKU: HB927207
Price: 191 USD
Availability: InStock

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概要
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データシート
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概要

カタログ番号	HB927207
種反応性	Human
アプリケーション	ELISA, FCM, IHC, WB
宿主種	Human
クローン性	Monoclonal
アイソタイプ	IgG2, kappa
クローンID	SAA0057
テスト済みアプリケーション	WB, FCM
ターゲット	PDGFR2, Platelet-derived growth factor receptor alpha, RHEPDGFRA, Alpha platelet-derived growth factor receptor, PDGFR-2, Platelet-derived growth factor alpha receptor, Platelet-derived growth factor receptor 2, PDGF-R-alpha, Alpha-type platelet-derived growth factor receptor, CD140a antigen, CD140 antigen-like family member A, PDGFRA, PDGFR-alpha, CD140a
エンドトキシンレベル	Please contact with the lab for this information.
純度	>95% as determined by SDS-PAGE.
精製	Protein A/G purified from cell culture supernatant.
アクセッション番号	P16234
形態	Liquid
保存バッファー	0.01M PBS pH 7.4 データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。
安定性と保存	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
背景	Platelet-derived growth factor receptor alpha (CD140a/PDGFRA) is a ~122 kDa protein. Tyrosine-protein kinase that acts as a cell-surface receptor for PDGFA, PDGFB and PDGFC and plays an essential role in the regulation of embryonic development, cell proliferation, survival and chemotaxis. Depending on the context, promotes or inhibits cell proliferation and cell migration. Plays an important role in the differentiation of bone marrow-derived mesenchymal stem cells. Required for normal skeleton development and cephalic closure during embryonic development. Required for normal development of the mucosa lining the gastrointestinal tract, and for recruitment of mesenchymal cells and normal development of intestinal villi.
注意事項	For research use only.

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Bioactivity

SEC-HPLC detection for Anti-Human CD140a/PDGFRA Antibody (ABS0556).
Flow Cytometry

Flow-cytometry using anti-human CD140a antibody. Human MG-63 cell line were stained with an irrelevant antibody (Green Histogram) or an anti-human CD140a antibody monoclonal antibody (Catalog # HB927207 , Red Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Western blot

Western blot analysis was performed using anti-CD140a/PDGFRA monoclonal antibody at 1μg/ml on various samples.
Lane 1: recombinant human CD140a/PDGFRA (Catalog No: HB927011)