Anti-Human PDGF R alpha Reference Antibody (Olaratumab, RUO) (HB927016)

Name: Anti-Human PDGF R alpha Reference Antibody (Olaratumab, RUO)
Brand: abinScience
SKU: HB927016
Price: 176 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.	HB927016
Description	Olaratumab (HB927016) is a research-grade recombinant antibody targeting CD140. Produced in mammalian cells with native-like glycosylation. Highlights ●Research Grade — For PK/PD studies, assay development, and ADA research. ●Native Glycosylation — Mammalian expression ensures native-like patterns.
Species reactivity	Human
Applications	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Host species	Human
Isotype	IgG1-kappa
Expression system	Mammalian cells
Clonality	Monoclonal
Target	PDGFR2, Platelet-derived growth factor receptor alpha, RHEPDGFRA, Alpha platelet-derived growth factor receptor, PDGFR-2, Platelet-derived growth factor alpha receptor, Platelet-derived growth factor receptor 2, PDGF-R-alpha, Alpha-type platelet-derived growth factor receptor, CD140a antigen, CD140 antigen-like family member A, PDGFRA, PDGFR-alpha, CD140a
Endotoxin level	Please contact the lab for this information.
Purity	>95% purity as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P16234
Form	Liquid
Storage buffer	0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	3G3, IMC-3G3, LY3012207, 1024603-93-7
Background	Platelet-derived growth factor receptor alpha (CD140a/PDGFRA) is a ~122 kDa protein. Tyrosine-protein kinase that acts as a cell-surface receptor for PDGFA, PDGFB and PDGFC and plays an essential role in the regulation of embryonic development, cell proliferation, survival and chemotaxis. Depending on the context, promotes or inhibits cell proliferation and cell migration. Plays an important role in the differentiation of bone marrow-derived mesenchymal stem cells. Required for normal skeleton development and cephalic closure during embryonic development. Required for normal development of the mucosa lining the gastrointestinal tract, and for recruitment of mesenchymal cells and normal development of intestinal villi.
Note	For research use only. Not suitable for clinical or therapeutic use.

Images

SEC-HPLC

SEC-HPLC detection for Research Grade Olaratumab.
Bioactivity

Detects CD140a/PDGFRA in indirect ELISAs.
Flow Cytometry

Flow-cytometry using anti-human PDGFRA antibody. MG-63 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human PDGFRA monoclonal antibody (Catalog HB927016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE

SDS-PAGE for Research Grade Olaratumab
Flow CytoMetry

Flow-cytometry using FITC anti-human CD140a antibody. MG-63 cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CD140a monoclonal antibody (Catalog: HB927016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using PE anti-human CD140a antibody. MG-63 cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD140a monoclonal antibody (Catalog: HB927016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using APC anti-human CD140a antibody. MG-63 cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD140a monoclonal antibody (Catalog: HB927016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.