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Anti-Human PDGF R alpha Recombinant Antibody (SAA0056) (HB927107)

Anti-Human PDGF R alpha Recombinant Antibody (SAA0056)
Anti-Human PDGF R alpha Recombinant Antibody (SAA0056)
Anti-Human PDGF R alpha Recombinant Antibody (SAA0056)
Anti-Human PDGF R alpha Recombinant Antibody (SAA0056)
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Overview
Catalog No.HB927107
Species reactivityHuman
ApplicationsFCM
Host speciesMouse
IsotypeIgG1, kappa
Clone IDSAA0056
Clonality Monoclonal
Target PDGFR2, Platelet-derived growth factor receptor alpha, RHEPDGFRA, Alpha platelet-derived growth factor receptor, PDGFR-2, Platelet-derived growth factor alpha receptor, Platelet-derived growth factor receptor 2, PDGF-R-alpha, Alpha-type platelet-derived growth factor receptor, CD140a antigen, CD140 antigen-like family member A, PDGFRA, PDGFR-alpha, CD140a
Endotoxin level Please contact with the lab for this information.
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Accession P16234
Form Liquid
Storage buffer 0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background

Platelet-derived growth factor receptor alpha (CD140a/PDGFRA) is a ~122 kDa protein. Tyrosine-protein kinase that acts as a cell-surface receptor for PDGFA, PDGFB and PDGFC and plays an essential role in the regulation of embryonic development, cell proliferation, survival and chemotaxis. Depending on the context, promotes or inhibits cell proliferation and cell migration. Plays an important role in the differentiation of bone marrow-derived mesenchymal stem cells. Required for normal skeleton development and cephalic closure during embryonic development. Required for normal development of the mucosa lining the gastrointestinal tract, and for recruitment of mesenchymal cells and normal development of intestinal villi.

Note For research use only.
Images
  • Anti-Human PDGF R alpha Recombinant Antibody (SAA0056)

    Flow Cytometry

    Flow-cytometry using anti-human CD140a antibody.CD140a Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD140a antibody monoclonal antibody (Catalog # HB927107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

  • Anti-Human PDGF R alpha Recombinant Antibody (SAA0056)

    Flow Cytometry

    Flow-cytometry using anti-human CD140a antibody.MG-63 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD140a antibody monoclonal antibody (Catalog # HB927107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

References
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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