Anti-Human MCAM/CD146 Recombinant Antibody (SAA0060) (HW484207)

Name: Anti-Human MCAM/CD146 Recombinant Antibody (SAA0060)
Brand: abinScience
SKU: HW484207
Price: 191 USD
Availability: InStock

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Aperçu

Numéro de catalogue	HW484207
Reactivité des espèces	Human
Applications	FCM
Espèce hôte	Human
Clonalité	Monoclonal
Isotype	IgG1, kappa
ID de clone	SAA0060
Applications testées	FCM
Cible	CD146, Melanoma cell adhesion molecule, S-endo 1 endothelial-associated antigen, Melanoma-associated antigen A32, MUC18, MCAM, Melanoma-associated antigen MUC18, Cell surface glycoprotein MUC18, Cell surface glycoprotein P1H12
Niveau d'endotoxines	Please contact with the lab for this information.
Pureté	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Numéro d'accès	P43121
Forme	Liquid
Tampon de stockage	0.01M PBS pH 7.4 Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Contexte	Cell surface glycoprotein MUC18 (CD146/MCAM) is a ~71 kDa protein. Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as a surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration.
Note	For research use only.

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Bioactivity

SEC-HPLC detection for Anti-Human CD146/MUC18/MCAM Antibody (ABS1541).
Flow Cytometry

Flow-cytometry using anti-human CD146 antibody. Human HeLa cell line were stained with an irrelevant antibody (Green Histogram) or an anti-human CD146 antibody monoclonal antibody (Catalog # HW484207 , Red Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.