Anti-Human MCAM/CD146 Reference Antibody (Abx-Ma1, RUO) (HW484026)

Name: Anti-Human MCAM/CD146 Reference Antibody (Abx-Ma1, RUO)
Brand: abinScience
SKU: HW484026
Price: 176 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.	HW484026
Species reactivity	Human
Applications	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Host species	Human
Isotype	IgG1
Clone ID	Abx-Ma1
Expression system	Mammalian cells
Clonality	Monoclonal
Target	CD146, Melanoma cell adhesion molecule, S-endo 1 endothelial-associated antigen, Melanoma-associated antigen A32, MUC18, MCAM, Melanoma-associated antigen MUC18, Cell surface glycoprotein MUC18, Cell surface glycoprotein P1H12
Endotoxin level	Please contact the lab for this information.
Purity	>95% purity as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P43121
Form	Liquid
Storage buffer	0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	C3.19.1, Abx-Ma1
Background	Cell surface glycoprotein MUC18 (CD146/MCAM) is a ~71 kDa protein. Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as a surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration.
References	1. Braeuer RR, et al. Transcriptional control of melanoma metastasis: the importance of the tumor microenvironment. Semin Cancer Biol. 2011 Apr;21(2):83-8. [HW484026]
Note	For research use only. Not suitable for clinical or therapeutic use.

Images

SEC-HPLC

SEC-HPLC detection for Research Grade Anti-Human CD146/MUC18/MCAM Antibody (Abx-Ma1).
Bioactivity

Detects CD146/MUC18/MCAM in indirect ELISAs.
SDS-PAGE

SDS-PAGE for Research Grade Anti-Human CD146/MUC18/MCAM Antibody (Abx-Ma1).
Flow CytoMetry

Flow-cytometry using APC anti-human CD146 antibody. Hela cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD146 monoclonal antibody (Catalog: HW484026, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using FITC anti-human CD146 antibody. Hela cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CD146 monoclonal antibody (Catalog: HW484026, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using PE anti-human CD146 antibody. Hela cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD146 monoclonal antibody (Catalog: HW484026, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.