Anti-Human CD156c/ADAM10 Recombinant Antibody (11G2) (HT182107)

Name: Anti-Human CD156c/ADAM10 Recombinant Antibody (11G2)
Brand: abinScience
SKU: HT182107
Price: 191 USD
Availability: InStock

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100ug

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Datasheet
SDS

Vista general

Número de catálogo	HT182107
Reactividad de especies	Human
Aplicaciones	FCM, WB
Especie huésped	Mouse
Clonalidad	Monoclonal
Isotipo	IgG1, kappa
ID de clon	11G2
Objetivo	ADAM 10, Mammalian disintegrin-metalloprotease, CD156c, MADM, ADAM10, Kuzbanian protein homolog, KUZ, CDw156, Disintegrin and metalloproteinase domain-containing protein 10
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>95% as determined by SDS-PAGE.
Purificación	Protein A/G purified from cell culture supernatant.
Número de acceso	O14672
Forma	Liquid
Buffer de almacenamiento	0.01M PBS pH 7.4 Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Fondo	Disintegrin and metalloproteinase domain-containing protein 10 (CD156c/ADAM10) is a ~84 kDa protein. Transmembrane metalloprotease which mediates the ectodomain shedding of a myriad of transmembrane proteins, including adhesion proteins, growth factor precursors and cytokines being essential for development and tissue homeostasis. Associates with six members of the tetraspanin superfamily TspanC8 which regulate its exit from the endoplasmic reticulum and its substrate selectivity. Cleaves the membrane-bound precursor of TNF at '76-Ala-\|-Val-77' to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth-like factor, ephrin-A2, CD44, CDH2 and for constitutive and regulated alpha-secretase cleavage of amyloid precursor protein (APP). 1. Lemjabbar, H. et al. (2002) Nature medicine 8, 41-6. PMID: 11786905 2. Gutwein, P. et al. (2003) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 17, 292-4. PMID: 12475894 3. Rabquer, BJ. et al. (2010) Journal of immunology (Baltimore, Md. : 1950) 185, 1777-85. PMID: 20592283 4. Kleinberger, G. et al. (2014) Science translational medicine 6, 243ra86. PMID: 24990881 5. Jouannet, S. et al. (2016) Cellular and molecular life sciences : CMLS 73, 1895-915. PMID: 26686862 6. Reyat, JS. et al. (2017) Journal of immunology (Baltimore, Md. : 1950) 199, 666-676. PMID: 28600292 7. Eschenbrenner, E. et al. (2020) Life science alliance 3. PMID: 31792032 8. Lipper, CH. et al. (2022) Structure (London, England : 1993) 30, 206-214.e4. PMID: 34739841 9. Lipper, CH. et al. (2023) Cell 186, 3632-3641.e10. PMID: 37516108
Nota	For research use only.

Imágenes

Flow-cytometry

Flow-cytometry using anti-human CD156c antibody.MCF7 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD156c antibody monoclonal antibody (Catalog # HT182107 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry

Flow-cytometry using anti-human CD156c antibody.THP-1 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD156c antibody monoclonal antibody (Catalog # HT182107 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry

Flow-cytometry using anti-human CD156c antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD156c antibody monoclonal antibody (Catalog # HT182107 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry

Flow-cytometry using anti-human CD156c antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD156c antibody monoclonal antibody (Catalog # HT182107 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Western blot

Western blot analysis was performed using anti-CD156c/ADAM10 monoclonal antibody at 1μg/ml on various samples.
Lane 1: recombinant human CD156c/ADAM10 (Catalog No: HT182012), 50ng
Lane 2: recombinant human CD156c/ADAM10 (Catalog No: HT182012), 30ng

Referencias

Datasheet

Anti-Human CD156c/ADAM10 Recombinant Antibody (11G2).pdf

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