Anti-CD156c/ADAM10 Polyclonal Antibody (HT182014)

Name: Anti-CD156c/ADAM10 Polyclonal Antibody
Brand: abinScience
SKU: HT182014
Price: 168 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HT182014

Description

Anti-CD156c/ADAM10 Polyclonal Antibody (HT182014) is a rabbit polyclonal antibody detecting CD156c in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Pig.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CD156c/ADAM10 (Thr214-Ser504).

Target

ADAM 10, Mammalian disintegrin-metalloprotease, CD156c, MADM, ADAM10, Kuzbanian protein homolog, KUZ, CDw156, Disintegrin and metalloproteinase domain-containing protein 10

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

O14672

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Disintegrin and metalloproteinase domain-containing protein 10 (CD156c/ADAM10) is a ~84 kDa protein. Transmembrane metalloprotease which mediates the ectodomain shedding of a myriad of transmembrane proteins, including adhesion proteins, growth factor precursors and cytokines being essential for development and tissue homeostasis. Associates with six members of the tetraspanin superfamily TspanC8 which regulate its exit from the endoplasmic reticulum and its substrate selectivity. Cleaves the membrane-bound precursor of TNF at '76-Ala-|-Val-77' to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth-like factor, ephrin-A2, CD44, CDH2 and for constitutive and regulated alpha-secretase cleavage of amyloid precursor protein (APP).

1. Lemjabbar, H. et al. (2002) Nature medicine 8, 41-6. PMID: 11786905

2. Gutwein, P. et al. (2003) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 17, 292-4. PMID: 12475894

3. Rabquer, BJ. et al. (2010) Journal of immunology (Baltimore, Md. : 1950) 185, 1777-85. PMID: 20592283

4. Kleinberger, G. et al. (2014) Science translational medicine 6, 243ra86. PMID: 24990881

5. Jouannet, S. et al. (2016) Cellular and molecular life sciences : CMLS 73, 1895-915. PMID: 26686862

6. Reyat, JS. et al. (2017) Journal of immunology (Baltimore, Md. : 1950) 199, 666-676. PMID: 28600292

7. Eschenbrenner, E. et al. (2020) Life science alliance 3. PMID: 31792032

8. Lipper, CH. et al. (2022) Structure (London, England : 1993) 30, 206-214.e4. PMID: 34739841

9. Lipper, CH. et al. (2023) Cell 186, 3632-3641.e10. PMID: 37516108

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-CD156c/ADAM10 polyclonal antibody at 1ug/mL on recombinant CD156c/ADAM10 (Catalog No. HT182012).

References

Datasheet