Anti-Human CD156c/ADAM10 Recombinant Antibody (11G2) (HT182107)

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Datasheet

Overview

Catalog No.	HT182107
Description	Anti-Human CD156c/ADAM10 Antibody (11G2) [11G2] (HT182107) is a mouse monoclonal antibody detecting CD156c in FCM, WB. Suitable for Human. Highlights ●Flow Cytometry — Validated for flow cytometry applications.
Species reactivity	Human
Applications	FCM, WB
Host species	Mouse
Isotype	IgG1, kappa
Clone ID	11G2
Clonality	Monoclonal
Target	ADAM 10, Mammalian disintegrin-metalloprotease, CD156c, MADM, ADAM10, Kuzbanian protein homolog, KUZ, CDw156, Disintegrin and metalloproteinase domain-containing protein 10
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	O14672
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background	Disintegrin and metalloproteinase domain-containing protein 10 (CD156c/ADAM10) is a ~84 kDa protein. Transmembrane metalloprotease which mediates the ectodomain shedding of a myriad of transmembrane proteins, including adhesion proteins, growth factor precursors and cytokines being essential for development and tissue homeostasis. Associates with six members of the tetraspanin superfamily TspanC8 which regulate its exit from the endoplasmic reticulum and its substrate selectivity. Cleaves the membrane-bound precursor of TNF at '76-Ala-\|-Val-77' to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth-like factor, ephrin-A2, CD44, CDH2 and for constitutive and regulated alpha-secretase cleavage of amyloid precursor protein (APP). 1. Lemjabbar, H. et al. (2002) Nature medicine 8, 41-6. PMID: 11786905 2. Gutwein, P. et al. (2003) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 17, 292-4. PMID: 12475894 3. Rabquer, BJ. et al. (2010) Journal of immunology (Baltimore, Md. : 1950) 185, 1777-85. PMID: 20592283 4. Kleinberger, G. et al. (2014) Science translational medicine 6, 243ra86. PMID: 24990881 5. Jouannet, S. et al. (2016) Cellular and molecular life sciences : CMLS 73, 1895-915. PMID: 26686862 6. Reyat, JS. et al. (2017) Journal of immunology (Baltimore, Md. : 1950) 199, 666-676. PMID: 28600292 7. Eschenbrenner, E. et al. (2020) Life science alliance 3. PMID: 31792032 8. Lipper, CH. et al. (2022) Structure (London, England : 1993) 30, 206-214.e4. PMID: 34739841 9. Lipper, CH. et al. (2023) Cell 186, 3632-3641.e10. PMID: 37516108
Note	For research use only.

Images

Flow-cytometry

Flow-cytometry using anti-human CD156c antibody.MCF7 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD156c antibody monoclonal antibody (Catalog # HT182107 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry

Flow-cytometry using anti-human CD156c antibody.THP-1 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD156c antibody monoclonal antibody (Catalog # HT182107 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry

Flow-cytometry using anti-human CD156c antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD156c antibody monoclonal antibody (Catalog # HT182107 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry

Flow-cytometry using anti-human CD156c antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD156c antibody monoclonal antibody (Catalog # HT182107 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Western blot

Western blot analysis was performed using anti-CD156c/ADAM10 monoclonal antibody at 1μg/ml on various samples.
Lane 1: recombinant human CD156c/ADAM10 (Catalog No: HT182012), 50ng
Lane 2: recombinant human CD156c/ADAM10 (Catalog No: HT182012), 30ng