







| Número de catálogo | HC298107 |
|---|---|
| Reactividad de especies | Human |
| Aplicaciones | ELISA, FCM |
| Especie huésped | Human |
| Clonalidad | Monoclonal |
| Isotipo | IgG1, kappa |
| ID de clon | SAA0065 |
| Objetivo | Selectin P ligand, PSGL-1, SELPLG, CD162, P-selectin glycoprotein ligand 1 |
| Nivel de endotoxinas | Please contact with the lab for this information. |
| Pureza | >95% as determined by SDS-PAGE. |
| Purificación | Protein A/G purified from cell culture supernatant. |
| Número de acceso | Q14242 |
| Forma | Liquid |
| Buffer de almacenamiento | 0.01M PBS pH 7.4 Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote. |
| Estabilidad y almacenamiento | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Fondo | P-selectin glycoprotein ligand 1 (CD162/SELPLG) is a ~43 kDa protein. A SLe(x)-type proteoglycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. Critical for the initial leukocyte capture. |
| Nota | For research use only. |

SEC-HPLC detection for Anti-Human CD162/SELPLG Antibody (ABS0199).

Flow-cytometry using anti-human CD162 antibody.Jurkat cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD162 antibody monoclonal antibody (Catalog # HC298107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using anti-human CD162 antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD162 antibody monoclonal antibody (Catalog # HC298107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using anti-human CD162 antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD162 antibody monoclonal antibody (Catalog # HC298107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.




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