Anti-Human Fc gamma RIIIA/CD16a Recombinant Antibody (SAA2437) (HY386033)

Name: Anti-Human Fc gamma RIIIA/CD16a Recombinant Antibody (SAA2437)
Brand: abinScience
SKU: HY386033
Price: 126 USD
Availability: InStock

データシート製品共有情報コピー

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20ug
50ug
100ug
1mg

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概要
イメージ
参考文献
データシート
SDS

概要

カタログ番号

HY386033

種反応性

Human

アプリケーション

ELISA, FACS, Functional assay

宿主種

Mouse

クローン性

Monoclonal

アイソタイプ

IgG2a, lambda

クローンID

SAA2437

テスト済みアプリケーション

FCM

ターゲット

Low affinity immunoglobulin gamma Fc region receptor III-A, IgG Fc receptor III-A, CD16-II, CD16a antigen, Fc-gamma RIII-alpha, Fc-gamma RIII, Fc-gamma RIIIa, FcRIII, FcRIIIa, FcgammaRIIIA, FcR-10, IgG Fc receptor III-2, CD16a, FCGR3A, CD16A, FCG3, FCGR3, IGFR3

エンドトキシンレベル

Please contact with the lab for this information.

純度

>95% as determined by SDS-PAGE.

精製

Protein A/G purified from cell culture supernatant.

アクセッション番号

P08637

形態

Liquid

保存バッファー

0.01M PBS pH 7.4

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

製品使用情報

アプリケーション	希釈
ELISA	1:2000-1:20000

安定性と保存

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

背景

Low affinity immunoglobulin gamma Fc region receptor III-A (CD16a) is a ~29 kDa protein. Receptor for the invariable Fc fragment of immunoglobulin gamma (IgG). Optimally activated upon binding of clustered antigen-IgG complexes displayed on cell surfaces, triggers lysis of antibody-coated cells, a process known as antibody-dependent cellular cytotoxicity (ADCC). Does not bind free monomeric IgG, thus avoiding inappropriate effector cell activation in the absence of antigenic trigger. Mediates IgG effector functions on natural killer (NK) cells. Binds antigen-IgG complexes generated upon infection and triggers NK cell-dependent cytokine production and degranulation to limit viral load and propagation.

1. Hezareh, M. et al. (2001) Journal of virology 75, 12161-8. PMID: 11711607

2. Ferrara, C. et al. (2011) Proceedings of the National Academy of Sciences of the United States of America 108, 12669-74. PMID: 21768335

3. Mizushima, T. et al. (2011) Genes to cells : devoted to molecular & cellular mechanisms 16, 1071-80. PMID: 22023369

4. DiLillo, DJ. et al. (2014) Nature medicine 20, 143-51. PMID: 24412922

5. Lee, J. et al. (2015) Immunity 42, 431-42. PMID: 25786175

6. Jing, Y. et al. (2015) PloS one 10, e0121788. PMID: 25816339

7. Blázquez-Moreno, A. et al. (2017) Proceedings of the National Academy of Sciences of the United States of America 114, E5645-E5654. PMID: 28652325

8. de Haas, M. et al. (1996) Journal of immunology (Baltimore, Md. : 1950) 156, 2948-55. PMID: 8609432

9. Koene, HR. et al. (1997) Blood 90, 1109-14. PMID: 9242542

注意事項

For research use only

Isotype Control

Mouse IgG2a (Fc Silence) Recombinant Isotype Control (MOPC 173) (MF533023)

イメージ

SDS-PAGE

SDS-PAGE for Anti-Human CD16a/FCGR3A Antibody (SAA2437)
Flow cytometry

Flow-cytometry APC using anti-human CD16A antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an APC anti-human CD16A monoclonal antibody (Catalog HY386033) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow cytometry

Flow-cytometry PE using anti-human CD16A antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an PE anti-human CD16A monoclonal antibody (Catalog HY386033) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow cytometry

Flow-cytometry FITC using anti-human CD16A antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an FITC anti-human CD16A monoclonal antibody (Catalog HY386033) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.