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Anti-Human CD16a/FCGR3A Recombinant Antibody (NM3E2), PerCP (HY386247)

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Anti-Human CD16a/FCGR3A Recombinant Antibody (NM3E2), PerCP

Anti-Human CD16a/FCGR3A Recombinant Antibody (NM3E2), PerCP

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Overview

Catalog No.	HY386247
Description	Anti-Human CD16a/FCGR3A Antibody (NM3E2), PerCP [NM3E2] (HY386247) is a human monoclonal antibody detecting FCGR3A in FCM. Suitable for Human. Highlights ●Flow Cytometry — Validated for flow cytometry applications. ●Fluorescent Label — Direct conjugate for streamlined workflows.
Species reactivity	Human
Applications	FCM
Host species	Human
Isotype	IgG1, kappa
Clone ID	NM3E2
Conjugation	PerCP
Clonality	Monoclonal
Target	CD16a, CD16A, FCGR3A, Fc-gamma RIII-alpha, FcRIIIa, IgG Fc receptor III-2, Low affinity immunoglobulin gamma Fc region receptor III-A, IGFR3, CD16a antigen, Fc-gamma RIII, FCGR3, Fc-gamma RIIIa, FCG3, FcRIII, FcR-10
Endotoxin level	Please contact with the lab for this information.
Accession	P08637
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Store at 4°C for 12 months. Protect from light. Do not freeze.
Background	Low affinity immunoglobulin gamma Fc region receptor III-A (CD16a/FCGR3A) is a ~29 kDa protein. Receptor for the invariable Fc fragment of immunoglobulin gamma (IgG). Optimally activated upon binding of clustered antigen-IgG complexes displayed on cell surfaces, triggers lysis of antibody-coated cells, a process known as antibody-dependent cellular cytotoxicity (ADCC). Does not bind free monomeric IgG, thus avoiding inappropriate effector cell activation in the absence of antigenic trigger. Mediates IgG effector functions on natural killer (NK) cells. Binds antigen-IgG complexes generated upon infection and triggers NK cell-dependent cytokine production and degranulation to limit viral load and propagation. 1. Hezareh, M. et al. (2001) Journal of virology 75, 12161-8. PMID: 11711607 2. Ferrara, C. et al. (2011) Proceedings of the National Academy of Sciences of the United States of America 108, 12669-74. PMID: 21768335 3. Mizushima, T. et al. (2011) Genes to cells : devoted to molecular & cellular mechanisms 16, 1071-80. PMID: 22023369 4. DiLillo, DJ. et al. (2014) Nature medicine 20, 143-51. PMID: 24412922 5. Lee, J. et al. (2015) Immunity 42, 431-42. PMID: 25786175 6. Jing, Y. et al. (2015) PloS one 10, e0121788. PMID: 25816339 7. Blázquez-Moreno, A. et al. (2017) Proceedings of the National Academy of Sciences of the United States of America 114, E5645-E5654. PMID: 28652325 8. de Haas, M. et al. (1996) Journal of immunology (Baltimore, Md. : 1950) 156, 2948-55. PMID: 8609432 9. Koene, HR. et al. (1997) Blood 90, 1109-14. PMID: 9242542
Note	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

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Flow cytometry

Flow-cytometry PerCP using anti-human CD16A antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an PerCP anti-human CD16A monoclonal antibody (Catalog HY386247) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet

Anti-Human CD16a/FCGR3A Recombinant Antibody (NM3E2), PerCP.pdf

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Anti-Human CD16a/FCGR3A Recombinant Antibody (NM3E2), PerCP