Anti-Human Fc gamma RIIIA/CD16a Recombinant Antibody (SAA2437) (HY386033)

Name: Anti-Human Fc gamma RIIIA/CD16a Recombinant Antibody (SAA2437)
Brand: abinScience
SKU: HY386033
Price: 126 USD
Availability: InStock

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Цена(USD):

Спецификация:

20ug
50ug
100ug
1mg

Количество:

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Обзор
Изображения
Ссылки
Даташит
SDS

Обзор

Каталожный номер

HY386033

Реактивность видов

Human

Применения

ELISA, FACS, Functional assay

Хозяинский вид

Mouse

Клоональность

Monoclonal

Изотип

IgG2a, lambda

ID клона

SAA2437

Проверенные приложения

FCM

Цель

Low affinity immunoglobulin gamma Fc region receptor III-A, IgG Fc receptor III-A, CD16-II, CD16a antigen, Fc-gamma RIII-alpha, Fc-gamma RIII, Fc-gamma RIIIa, FcRIII, FcRIIIa, FcgammaRIIIA, FcR-10, IgG Fc receptor III-2, CD16a, FCGR3A, CD16A, FCG3, FCGR3, IGFR3

Уровень эндотоксинов

Please contact with the lab for this information.

Чистота

>95% as determined by SDS-PAGE.

Очистка

Protein A/G purified from cell culture supernatant.

Номер доступа

P08637

Форма

Liquid

Буфер хранения

0.01M PBS pH 7.4

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта

Применение	Разбавление
ELISA	1:2000-1:20000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Фон

Low affinity immunoglobulin gamma Fc region receptor III-A (CD16a) is a ~29 kDa protein. Receptor for the invariable Fc fragment of immunoglobulin gamma (IgG). Optimally activated upon binding of clustered antigen-IgG complexes displayed on cell surfaces, triggers lysis of antibody-coated cells, a process known as antibody-dependent cellular cytotoxicity (ADCC). Does not bind free monomeric IgG, thus avoiding inappropriate effector cell activation in the absence of antigenic trigger. Mediates IgG effector functions on natural killer (NK) cells. Binds antigen-IgG complexes generated upon infection and triggers NK cell-dependent cytokine production and degranulation to limit viral load and propagation.

1. Hezareh, M. et al. (2001) Journal of virology 75, 12161-8. PMID: 11711607

2. Ferrara, C. et al. (2011) Proceedings of the National Academy of Sciences of the United States of America 108, 12669-74. PMID: 21768335

3. Mizushima, T. et al. (2011) Genes to cells : devoted to molecular & cellular mechanisms 16, 1071-80. PMID: 22023369

4. DiLillo, DJ. et al. (2014) Nature medicine 20, 143-51. PMID: 24412922

5. Lee, J. et al. (2015) Immunity 42, 431-42. PMID: 25786175

6. Jing, Y. et al. (2015) PloS one 10, e0121788. PMID: 25816339

7. Blázquez-Moreno, A. et al. (2017) Proceedings of the National Academy of Sciences of the United States of America 114, E5645-E5654. PMID: 28652325

8. de Haas, M. et al. (1996) Journal of immunology (Baltimore, Md. : 1950) 156, 2948-55. PMID: 8609432

9. Koene, HR. et al. (1997) Blood 90, 1109-14. PMID: 9242542

Примечание

For research use only

Isotype Control

Mouse IgG2a (Fc Silence) Recombinant Isotype Control (MOPC 173) (MF533023)

Изображения

SDS-PAGE

SDS-PAGE for Anti-Human CD16a/FCGR3A Antibody (SAA2437)
Flow cytometry

Flow-cytometry APC using anti-human CD16A antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an APC anti-human CD16A monoclonal antibody (Catalog HY386033) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow cytometry

Flow-cytometry PE using anti-human CD16A antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an PE anti-human CD16A monoclonal antibody (Catalog HY386033) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Flow cytometry

Flow-cytometry FITC using anti-human CD16A antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an FITC anti-human CD16A monoclonal antibody (Catalog HY386033) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.