Anti-Human CD2 Recombinant Antibody (G11) (HY113107)

Name: Anti-Human CD2 Recombinant Antibody (G11)
Brand: abinScience
SKU: HY113107
Price: 118 USD
Availability: InStock

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Vista general

Número de catálogo	HY113107
Reactividad de especies	Human
Aplicaciones	FCM
Especie huésped	Mouse
Clonalidad	Monoclonal
Isotipo	IgG2a, kappa
ID de clon	G11
Aplicaciones probadas	WB, FCM
Objetivo	Erythrocyte receptor, SRBC, T-cell surface antigen CD2, Rosette receptor, CD2, LFA-3 receptor, T-cell surface antigen T11/Leu-5, LFA-2
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>95% as determined by SDS-PAGE.
Purificación	Protein A/G purified from cell culture supernatant.
Número de acceso	P06729
Forma	Liquid
Buffer de almacenamiento	0.01M PBS pH 7.4 Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Fondo	T-cell surface antigen CD2 (LFA-2/OX-34) is a ~39 kDa protein. CD2 interacts with lymphocyte function-associated antigen CD58 (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.
Nota	For research use only.

Imágenes

Bioactivity

Detects CD2 in indirect ELISAs.
Flow Cytometry

Flow-cytometry using anti-human CD2 antibody. Human Jurkat cell line were stained with an irrelevant antibody (Green Histogram) or an anti-human CD2 antibody monoclonal antibody (Catalog # HY113107 , Red Histogram) at a concentration of 20 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a CoraLite488 conjugated goat anti-mouse antibody (Catalog # MF690314) and cells analysed on a NovoCyte Flow Cytometer.
Western blot

Western blot analysis was performed using anti-CD2 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human CD2 (Catalog No: HY113011)
Lane 2: negative control