Anti-Human CD2 Recombinant Antibody (G11) (HY113107)

Name: Anti-Human CD2 Recombinant Antibody (G11)
Brand: abinScience
SKU: HY113107
Price: 118 USD
Availability: InStock

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Изображения
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Даташит
SDS

Обзор

Каталожный номер	HY113107
Реактивность видов	Human
Применения	FCM
Хозяинский вид	Mouse
Клоональность	Monoclonal
Изотип	IgG2a, kappa
ID клона	G11
Проверенные приложения	WB, FCM
Цель	Erythrocyte receptor, SRBC, T-cell surface antigen CD2, Rosette receptor, CD2, LFA-3 receptor, T-cell surface antigen T11/Leu-5, LFA-2
Уровень эндотоксинов	Please contact with the lab for this information.
Чистота	>95% as determined by SDS-PAGE.
Очистка	Protein A/G purified from cell culture supernatant.
Номер доступа	P06729
Форма	Liquid
Буфер хранения	0.01M PBS pH 7.4 Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Устойчивость и хранение	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Фон	T-cell surface antigen CD2 (LFA-2/OX-34) is a ~39 kDa protein. CD2 interacts with lymphocyte function-associated antigen CD58 (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.
Примечание	For research use only.

Изображения

Bioactivity

Detects CD2 in indirect ELISAs.
Flow Cytometry

Flow-cytometry using anti-human CD2 antibody. Human Jurkat cell line were stained with an irrelevant antibody (Green Histogram) or an anti-human CD2 antibody monoclonal antibody (Catalog # HY113107 , Red Histogram) at a concentration of 20 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a CoraLite488 conjugated goat anti-mouse antibody (Catalog # MF690314) and cells analysed on a NovoCyte Flow Cytometer.
Western blot

Western blot analysis was performed using anti-CD2 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human CD2 (Catalog No: HY113011)
Lane 2: negative control