Anti-Human DNAM-1/CD226 Recombinant Antibody (SAA0074), APC (HS737137)

Name: Anti-Human DNAM-1/CD226 Recombinant Antibody (SAA0074), APC
Brand: abinScience
SKU: HS737137
Price: 290 USD
Availability: InStock

データシート製品共有情報コピー

価格(USD):

スペック:

50T
100T

数量:

お問い合わせ

概要
イメージ
参考文献
データシート
SDS

概要

カタログ番号	HS737137
種反応性	Human
アプリケーション	FCM
宿主種	Mouse
クローン性	Monoclonal
アイソタイプ	IgG1, kappa
クローンID	SAA0074
抱合	APC
ターゲット	DNAX accessory molecule 1, DNAM-1, DNAM1, CD226 antigen, CD226
エンドトキシンレベル	Please contact with the lab for this information.
関連製品	Mouse IgG1, kappa Recombinant Isotype Control Antibody (MOPC-21), APC
アクセッション番号	Q15762
形態	Liquid
保存バッファー	0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。
安定性と保存	Store at 4°C for 12 months. Protect from light. Do not freeze.
背景	CD226 antigen (DNAM-1) is a ~38 kDa protein. Cell surface receptor that plays an important role in the immune system, particularly in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T-cells and NK cells. Functions as a costimulatory receptor upon recognition of target cells, such as virus-infected or tumor cells. Upon binding to its ligands PVR/CD155 or NECTIN2/CD112 on target cells, promotes the cytotoxic activity of NK cells and CTLs, enhancing their ability to kill these cells. Mechanistically, phosphorylation by Src kinases such as LYN of FYN, enables binding to adapter GRB2, leading to activation of VAV1, PI3K and PLCG1. Promotes also activation of kinases ERK and AKT, as well as calcium fluxes. 1. Shibuya, A. et al. (1996) Immunity 4, 573-81. PMID: 8673704 2. Shibuya, A. et al. (1998) Journal of immunology (Baltimore, Md. : 1950) 161, 1671-6. PMID: 9712030 3. Zhu, Y. et al. (2016) The Journal of experimental medicine 213, 167-76. PMID: 26755705 4. Deuss, FA. et al. (2019) The Journal of biological chemistry 294, 12534-12546. PMID: 31253644 5. Wang, H. et al. (2019) Proceedings of the National Academy of Sciences of the United States of America 116, 988-996. PMID: 30591568
注意事項	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

イメージ

Flow CytoMetry

Flow-cytometry using APC anti-human CD226 antibody. HEL cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD226 monoclonal antibody (Catalog: HS737137, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.

参考文献

データシート