Anti-Human DNAM-1/CD226 Polyclonal Antibody (HS737014)

Name: Anti-Human DNAM-1/CD226 Polyclonal Antibody
Brand: abinScience
SKU: HS737014
Price: 96 USD
Availability: InStock

Datasheet Compartir producto Copiar información

Precio(USD):

Especificación:

20ug
50ug
100ug

Número:

Contáctenos

Vista general
Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo

HS737014

Descripción

Anti-Human CD226/DNAM-1 Polyclonal Antibody (HS737014) is a rabbit polyclonal antibody detecting DNAM-1 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Reactividad de especies

Human

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant Human CD226/DNAM-1 (Glu19-Glu242).

Objetivo

CD226 antigen, DNAX accessory molecule 1, DNAM-1, CD226, CD226, DNAM1

Purificación

Purified by antigen affinity column.

Número de acceso

Q15762

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

CD226 antigen (DNAM-1) is a ~38 kDa protein. Cell surface receptor that plays an important role in the immune system, particularly in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T-cells and NK cells. Functions as a costimulatory receptor upon recognition of target cells, such as virus-infected or tumor cells. Upon binding to its ligands PVR/CD155 or NECTIN2/CD112 on target cells, promotes the cytotoxic activity of NK cells and CTLs, enhancing their ability to kill these cells. Mechanistically, phosphorylation by Src kinases such as LYN of FYN, enables binding to adapter GRB2, leading to activation of VAV1, PI3K and PLCG1. Promotes also activation of kinases ERK and AKT, as well as calcium fluxes.

1. Shibuya, A. et al. (1996) Immunity 4, 573-81. PMID: 8673704

2. Shibuya, A. et al. (1998) Journal of immunology (Baltimore, Md. : 1950) 161, 1671-6. PMID: 9712030

3. Zhu, Y. et al. (2016) The Journal of experimental medicine 213, 167-76. PMID: 26755705

4. Deuss, FA. et al. (2019) The Journal of biological chemistry 294, 12534-12546. PMID: 31253644

5. Wang, H. et al. (2019) Proceedings of the National Academy of Sciences of the United States of America 116, 988-996. PMID: 30591568

Nota

For research use only.

Imágenes

Western blot

Western blot analysis was performed using anti-CD226 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Jurkat cell lysate
Lane 2: PC-12 cell lysate
Lane 3: Hela cell lysate
Lane 4: Mouse spleen lysate
Lane 5: Mouse lung lysate
Lane 6: Rat spleen lysate
Lane 7: Rat lung lysate