Anti-Human DNAM-1/CD226 Polyclonal Antibody (HS737014)

Name: Anti-Human DNAM-1/CD226 Polyclonal Antibody
Brand: abinScience
SKU: HS737014
Price: 96 USD
Availability: InStock

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SDS

Aperçu

Numéro de catalogue

HS737014

Description

Anti-Human CD226/DNAM-1 Polyclonal Antibody (HS737014) is a rabbit polyclonal antibody detecting DNAM-1 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Reactivité des espèces

Human

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human CD226/DNAM-1 (Glu19-Glu242).

Cible

CD226 antigen, DNAX accessory molecule 1, DNAM-1, CD226, CD226, DNAM1

Purification

Purified by antigen affinity column.

Numéro d'accès

Q15762

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

CD226 antigen (DNAM-1) is a ~38 kDa protein. Cell surface receptor that plays an important role in the immune system, particularly in intercellular adhesion, lymphocyte signaling, cytotoxicity and lymphokine secretion mediated by cytotoxic T-cells and NK cells. Functions as a costimulatory receptor upon recognition of target cells, such as virus-infected or tumor cells. Upon binding to its ligands PVR/CD155 or NECTIN2/CD112 on target cells, promotes the cytotoxic activity of NK cells and CTLs, enhancing their ability to kill these cells. Mechanistically, phosphorylation by Src kinases such as LYN of FYN, enables binding to adapter GRB2, leading to activation of VAV1, PI3K and PLCG1. Promotes also activation of kinases ERK and AKT, as well as calcium fluxes.

1. Shibuya, A. et al. (1996) Immunity 4, 573-81. PMID: 8673704

2. Shibuya, A. et al. (1998) Journal of immunology (Baltimore, Md. : 1950) 161, 1671-6. PMID: 9712030

3. Zhu, Y. et al. (2016) The Journal of experimental medicine 213, 167-76. PMID: 26755705

4. Deuss, FA. et al. (2019) The Journal of biological chemistry 294, 12534-12546. PMID: 31253644

5. Wang, H. et al. (2019) Proceedings of the National Academy of Sciences of the United States of America 116, 988-996. PMID: 30591568

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-CD226 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Jurkat cell lysate
Lane 2: PC-12 cell lysate
Lane 3: Hela cell lysate
Lane 4: Mouse spleen lysate
Lane 5: Mouse lung lysate
Lane 6: Rat spleen lysate
Lane 7: Rat lung lysate