Anti-Human CD24 Recombinant Antibody (SWA11) (HB630107)

Name: Anti-Human CD24 Recombinant Antibody (SWA11)
Brand: abinScience
SKU: HB630107
Price: 196 USD
Availability: InStock

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Vista general

Número de catálogo	HB630107
Reactividad de especies	Human
Aplicaciones	FCM, IHC, IF, RIA
Especie huésped	Mouse
Clonalidad	Monoclonal
Isotipo	IgG1, kappa
ID de clon	SWA11
Aplicaciones probadas	WB, FCM
Objetivo	Signal transducer CD24, CD24, CD24A, Small cell lung carcinoma cluster 4 antigen
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>95% as determined by SDS-PAGE.
Purificación	Protein A/G purified from cell culture supernatant.
Número de acceso	P25063
Forma	Liquid
Buffer de almacenamiento	0.01M PBS pH 7.4 Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Fondo	Signal transducer CD24 is a ~8 kDa protein. May have a pivotal role in cell differentiation of different cell types. Signaling could be triggered by the binding of a lectin-like ligand to the CD24 carbohydrates, and transduced by the release of second messengers derived from the GPI-anchor. Modulates B-cell activation responses. Promotes AG-dependent proliferation of B-cells, and prevents their terminal differentiation into antibody-forming cells. In association with SIGLEC10 may be involved in the selective suppression of the immune response to danger-associated molecular patterns (DAMPs) such as HMGB1, HSP70 and HSP90. 1. Suzuki, T. et al. (2001) Journal of immunology (Baltimore, Md. : 1950) 166, 5567-77. PMID: 11313396
Nota	For research use only.

Imágenes

SEC-HPLC

SEC-HPLC detection for Anti-Human CD24 Antibody (SWA11).
Bioactivity

Detects CD24 in indirect ELISAs.
SDS-PAGE

SDS-PAGE for Anti-Human CD24 Antibody (SWA11)
Flow Cytometry

Flow-cytometry using anti-human CD24 antibody. Human SK-BR-3 cell line were stained with an irrelevant antibody (Green Histogram) or an anti-human CD24 antibody monoclonal antibody (Catalog # HB630107 , Red Histogram) at a concentration of 20 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a CoraLite488 conjugated goat anti-mouse antibody (Catalog # MF690314) and cells analysed on a NovoCyte Flow Cytometer.
Western blot

Western blot analysis was performed using anti-CD24 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human CD24 (Catalog No: HB630011)
Lane 2: negative control