Anti-Human CD24 Reference Antibody (ATG 031, RUO) (HB630016)

Name: Anti-Human CD24 Reference Antibody (ATG 031, RUO)
Brand: abinScience
SKU: HB630016
Price: 176 USD
Availability: InStock

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Overview

Catalog No.	HB630016
Species reactivity	Human
Applications	ELISA, Bioactivity: FACS, Functional assay, Research in vivo
Host species	Human
Isotype	IgG
Clone ID	ATG 031
Biological activity	ATG-031 potently binds to CD24-postive tumor cells, while showing no binding with parental HEK293 cells or hRBC [1]. ATG-031 blocks the interaction between CD24 and Siglec-10 and induces potent macrophage-dependent tumor cell phagocytosis [1]. ATG-031 induces a potent antibody-dependent cellular cytotoxicity against HT-29 tumor cells with an EC 50 of 0.27 nM [3]. ATG-031 (1-3 mg/kg, i.v., biweekly) significantly inhibits tumor growth and induces tumor regression in MC38-hCD24 mouse model [1]. ATG-031 exhibits potent antitumor activity in vivo as a single agent in syngeneic models such as MC38, CT26, and 4T1 [3]. ATG-031 demonstrates in vivo synergism with chemotherapies and checkpoint inhibitors [3]. ATG-031 (3 mg/kg, i.v., 2 doses) significantly increases T cell-associated immune response at the cellular and single-cell transcriptome levels in C57BL/6 mice bearing MC38-hCD24 tumors [3].
Expression system	Mammalian cells
Clonality	Monoclonal
Target	Signal transducer CD24, CD24, CD24A, Small cell lung carcinoma cluster 4 antigen
Endotoxin level	Please contact the lab for this information.
Purity	>95% purity as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	P25063
Form	Liquid
Storage buffer	0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	ATG-031, ATG031, ATG031
Background	Signal transducer CD24 is a ~8 kDa protein. May have a pivotal role in cell differentiation of different cell types. Signaling could be triggered by the binding of a lectin-like ligand to the CD24 carbohydrates, and transduced by the release of second messengers derived from the GPI-anchor. Modulates B-cell activation responses. Promotes AG-dependent proliferation of B-cells, and prevents their terminal differentiation into antibody-forming cells. In association with SIGLEC10 may be involved in the selective suppression of the immune response to danger-associated molecular patterns (DAMPs) such as HMGB1, HSP70 and HSP90. 1. Suzuki, T. et al. (2001) Journal of immunology (Baltimore, Md. : 1950) 166, 5567-77. PMID: 11313396
References	1. Peng Chen, et al., (2022). ATG-031, a first-in-class anti-CD24 antibody, showed potent preclinical anti-tumor efficacy by blocking “don’t-eat-me” signal. Journal for ImmunoTherapy of Cancer 2022. [HB630016] 2. Peng Chen, et al., Abstract 6641: ATG-031, a first-in-class humanized anti-CD24 antibody, demonstrates potent in vivo efficacy and repolarizes tumor-associated macrophages in the TME. Cancer Res 1 April 2023; 83 (7_Supplement): 6641. [HB630016] 3. Chen P, et al., 1337 The preclinical characterization and translational research of ATG-031, a first-in-class humanized anti-CD24 antibody, for the treatment of solid tumors and hematological malignanciesJournal for ImmunoTherapy of Cancer 2023;11 [HB630016]
Note	For research use only. Not suitable for clinical or therapeutic use.

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Anti-Human CD24 Reference Antibody (ATG 031, RUO).pdf

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Anti-Human CD24 Reference Antibody (ATG 031, RUO) (HB630016)

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