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Anti-Human CD30/TNFRSF8 Recombinant Antibody (SAA0011) (HB038207)

Anti-Human CD30/TNFRSF8 Recombinant Antibody (SAA0011)
Anti-Human CD30/TNFRSF8 Recombinant Antibody (SAA0011)
Anti-Human CD30/TNFRSF8 Recombinant Antibody (SAA0011)
Anti-Human CD30/TNFRSF8 Recombinant Antibody (SAA0011)
Anti-Human CD30/TNFRSF8 Recombinant Antibody (SAA0011)
Anti-Human CD30/TNFRSF8 Recombinant Antibody (SAA0011)
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Aperçu
Numéro de catalogueHB038207
Reactivité des espècesHuman
ApplicationsFCM
Espèce hôteMouse
ClonalitéMonoclonal
IsotypeIgG1, kappa
ID de cloneSAA0011
Applications testées FCM
Cible CD30L receptor, Ki-1 antigen, CD30, TNFRSF8, D1S166E, Tumor necrosis factor receptor superfamily member 8, Lymphocyte activation antigen CD30
Niveau d'endotoxines Please contact with the lab for this information.
Pureté >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Numéro d'accès P28908
Forme Liquid
Tampon de stockage 0.01M PBS pH 7.4

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Contexte

Tumor necrosis factor receptor superfamily member 8 (CD30/TNFRSF8) is a ~63 kDa protein. Receptor for TNFSF8/CD30L. May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF-kappa-B. CD30 is the therapeutic target of brentuximab vedotin (Adcetris).

1. Smith, CA. et al. (1993) Cell 73, 1349-60. PMID: 8391931
2. Aizawa, S. et al. (1997) The Journal of biological chemistry 272, 2042-5. PMID: 8999898
Note For research use only.
Images
  • Anti-Human CD30/TNFRSF8 Recombinant Antibody (SAA0011)

    Flow Cytometry

    Flow-cytometry using anti-human CD30 antibody.CD30 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD30 antibody monoclonal antibody (Catalog # HB038207 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

  • Anti-Human CD30/TNFRSF8 Recombinant Antibody (SAA0011)

    Flow Cytometry

    Flow-cytometry using anti-human CD30 antibody.Jurkat cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD30 antibody monoclonal antibody (Catalog # HB038207 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

  • Anti-Human CD30/TNFRSF8 Recombinant Antibody (SAA0011)

    Flow CytoMetry

    Flow-cytometry using anti-human CD30 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CD30 monoclonal antibody (Catalog: HB038207) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.

Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Recommandation

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