





| Numéro de catalogue | HB038207 |
|---|---|
| Reactivité des espèces | Human |
| Applications | FCM |
| Espèce hôte | Mouse |
| Clonalité | Monoclonal |
| Isotype | IgG1, kappa |
| ID de clone | SAA0011 |
| Applications testées | FCM |
| Cible | CD30L receptor, Ki-1 antigen, CD30, TNFRSF8, D1S166E, Tumor necrosis factor receptor superfamily member 8, Lymphocyte activation antigen CD30 |
| Niveau d'endotoxines | Please contact with the lab for this information. |
| Pureté | >95% as determined by SDS-PAGE. |
| Purification | Protein A/G purified from cell culture supernatant. |
| Numéro d'accès | P28908 |
| Forme | Liquid |
| Tampon de stockage | 0.01M PBS pH 7.4 Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot. |
| Stabilité et stockage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Contexte | Tumor necrosis factor receptor superfamily member 8 (CD30/TNFRSF8) is a ~63 kDa protein. Receptor for TNFSF8/CD30L. May play a role in the regulation of cellular growth and transformation of activated lymphoblasts. Regulates gene expression through activation of NF-kappa-B. CD30 is the therapeutic target of brentuximab vedotin (Adcetris). 1. Smith, CA. et al. (1993) Cell 73, 1349-60. PMID: 8391931 2. Aizawa, S. et al. (1997) The Journal of biological chemistry 272, 2042-5. PMID: 8999898 |
| Note | For research use only. |

Flow-cytometry using anti-human CD30 antibody.CD30 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD30 antibody monoclonal antibody (Catalog # HB038207 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using anti-human CD30 antibody.Jurkat cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD30 antibody monoclonal antibody (Catalog # HB038207 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using anti-human CD30 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CD30 monoclonal antibody (Catalog: HB038207) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.




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