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Anti-Human Fc gamma RIIB/CD32b Recombinant Antibody (6G08), APC (HB103237)

Anti-Human Fc gamma RIIB/CD32b Recombinant Antibody (6G08), APC
Anti-Human Fc gamma RIIB/CD32b Recombinant Antibody (6G08), APC
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Aperçu
Numéro de catalogueHB103237
Reactivité des espècesHuman
ApplicationsFCM
Espèce hôteMouse
ClonalitéMonoclonal
IsotypeIgG1, lambda
ID de clone6G08
ConjugaisonAPC
Applications testées FCM
Cible IGFR2, FCG2, Fc-gamma RII-b, CDw32, FcRII-b, IgG Fc receptor II-b, CD32, Fc-gamma-RIIb, Low affinity immunoglobulin gamma Fc region receptor II-b, FCGR2B
Niveau d'endotoxines Please contact with the lab for this information.
Produits associés Mouse IgG1 Recombinant Isotype Control Antibody (HyHEL-10), APC
Numéro d'accès P31994
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Stabilité et stockage Store at 4°C for 12 months. Protect from light. Do not freeze.
Contexte

Low affinity immunoglobulin gamma Fc region receptor II-b (CD32b/FCGR2B) is a ~34 kDa protein. Receptor for the Fc region of complexed or aggregated immunoglobulins gamma. Low affinity receptor. Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B-cells. Binding to this receptor results in down-modulation of previous state of cell activation triggered via antigen receptors on B-cells (BCR), T-cells (TCR) or via another Fc receptor. Isoform IIB1 fails to mediate endocytosis or phagocytosis.

Note For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.
Images
  • Anti-Human Fc gamma RIIB/CD32b Recombinant Antibody (6G08), APC

    Flow CytoMetry

    Flow-cytometry using APC anti-human CD32b antibody. Raji cells were stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CD32b monoclonal antibody (Catalog: HB103237, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
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Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
Recommandation

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