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Anti-Human Fc gamma RIIB/CD32b Recombinant Antibody (SAA0547) (HB103107)

Anti-Human Fc gamma RIIB/CD32b Recombinant Antibody (SAA0547)
Anti-Human Fc gamma RIIB/CD32b Recombinant Antibody (SAA0547)
Anti-Human Fc gamma RIIB/CD32b Recombinant Antibody (SAA0547)
Anti-Human Fc gamma RIIB/CD32b Recombinant Antibody (SAA0547)
Anti-Human Fc gamma RIIB/CD32b Recombinant Antibody (SAA0547)
Anti-Human Fc gamma RIIB/CD32b Recombinant Antibody (SAA0547)
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  • 100ug
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Vista general
Número de catálogoHB103107
Reactividad de especiesHuman
AplicacionesELISA, FCM, IHC, WB
Especie huéspedHuman
ClonalidadMonoclonal
IsotipoIgG1, kappa
ID de clonSAA0547
Aplicaciones probadas FCM
Objetivo IGFR2, FCG2, Fc-gamma RII-b, CDw32, FcRII-b, IgG Fc receptor II-b, CD32, Fc-gamma-RIIb, Low affinity immunoglobulin gamma Fc region receptor II-b, FCGR2B, RHD97901
Nivel de endotoxinas Please contact with the lab for this information.
Pureza >95% as determined by SDS-PAGE.
Purificación Protein A/G purified from cell culture supernatant.
Número de acceso P31994
Forma Liquid
Buffer de almacenamiento 0.01M PBS pH 7.4

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Fondo

Low affinity immunoglobulin gamma Fc region receptor II-b (CD32b/FCGR2B) is a ~34 kDa protein. Receptor for the Fc region of complexed or aggregated immunoglobulins gamma. Low affinity receptor. Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B-cells. Binding to this receptor results in down-modulation of previous state of cell activation triggered via antigen receptors on B-cells (BCR), T-cells (TCR) or via another Fc receptor. Isoform IIB1 fails to mediate endocytosis or phagocytosis.

Nota For research use only.
Imágenes
  • Anti-Human Fc gamma RIIB/CD32b Recombinant Antibody (SAA0547)

    SDS-PAGE

    SDS-PAGE for Anti-Human CD32b/FCGR2B Antibody (SAA0547)

  • Anti-Human Fc gamma RIIB/CD32b Recombinant Antibody (SAA0547)

    Bioactivity

    SEC-HPLC detection for Anti-Human CD32b/FCGR2B Antibody (ABS0184).

  • Anti-Human Fc gamma RIIB/CD32b Recombinant Antibody (SAA0547)

    Flow Cytometry

    Flow-cytometry using anti-human CD32b antibody.CD32b Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD32b antibody monoclonal antibody (Catalog # HB103107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.

Referencias
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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