Anti-Human Fc gamma RIIB/CD32b Recombinant Antibody (SAA0547) (HB103107)

Name: Anti-Human Fc gamma RIIB/CD32b Recombinant Antibody (SAA0547)
Brand: abinScience
SKU: HB103107
Price: 196 USD
Availability: InStock

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Aperçu

Numéro de catalogue	HB103107
Reactivité des espèces	Human
Applications	ELISA, FCM, IHC, WB
Espèce hôte	Human
Clonalité	Monoclonal
Isotype	IgG1, kappa
ID de clone	SAA0547
Applications testées	FCM
Cible	IGFR2, FCG2, Fc-gamma RII-b, CDw32, FcRII-b, IgG Fc receptor II-b, CD32, Fc-gamma-RIIb, Low affinity immunoglobulin gamma Fc region receptor II-b, FCGR2B, RHD97901
Niveau d'endotoxines	Please contact with the lab for this information.
Pureté	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Numéro d'accès	P31994
Forme	Liquid
Tampon de stockage	0.01M PBS pH 7.4 Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Contexte	Low affinity immunoglobulin gamma Fc region receptor II-b (CD32b/FCGR2B) is a ~34 kDa protein. Receptor for the Fc region of complexed or aggregated immunoglobulins gamma. Low affinity receptor. Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B-cells. Binding to this receptor results in down-modulation of previous state of cell activation triggered via antigen receptors on B-cells (BCR), T-cells (TCR) or via another Fc receptor. Isoform IIB1 fails to mediate endocytosis or phagocytosis.
Note	For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Human CD32b/FCGR2B Antibody (SAA0547)
Bioactivity

SEC-HPLC detection for Anti-Human CD32b/FCGR2B Antibody (ABS0184).
Flow Cytometry

Flow-cytometry using anti-human CD32b antibody.CD32b Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD32b antibody monoclonal antibody (Catalog # HB103107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.