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Anti-Human CD34 Recombinant Antibody (QBEND-10) (HB766207)

Anti-Human CD34 Recombinant Antibody (QBEND-10)
Anti-Human CD34 Recombinant Antibody (QBEND-10)
Anti-Human CD34 Recombinant Antibody (QBEND-10)
Anti-Human CD34 Recombinant Antibody (QBEND-10)
Anti-Human CD34 Recombinant Antibody (QBEND-10)
Anti-Human CD34 Recombinant Antibody (QBEND-10)
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Aperçu
Numéro de catalogueHB766207
Reactivité des espècesHuman
ApplicationsFCM
Espèce hôteMouse
ClonalitéMonoclonal
IsotypeIgG1, lambda
ID de cloneQBEND-10
Applications testées FCM
Cible CD34, Hematopoietic progenitor cell antigen CD34
Niveau d'endotoxines Please contact with the lab for this information.
Pureté >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Numéro d'accès P28906
Forme Liquid
Tampon de stockage 0.01M PBS pH 7.4

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Contexte

Hematopoietic progenitor cell antigen CD34 is a ~40 kDa protein. Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.

Note For research use only.
Images
  • Anti-Human CD34 Recombinant Antibody (QBEND-10)

    Bioactivity

    SEC-HPLC detection for Anti-Human CD34 Antibody (QBEND-10).

  • Anti-Human CD34 Recombinant Antibody (QBEND-10)

    Flow Cytometry

    Flow-cytometry using anti-human CD34 antibody.CD34 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD34 antibody monoclonal antibody (Catalog # HB766207 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

  • Anti-Human CD34 Recombinant Antibody (QBEND-10)

    Flow Cytometry

    Flow-cytometry using anti-human CD34 antibody.KG-1 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD34 antibody monoclonal antibody (Catalog # HB766207 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Recommandation

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