Anti-Human CD34 Recombinant Antibody (QBEND-10) (HB766207)

Name: Anti-Human CD34 Recombinant Antibody (QBEND-10)
Brand: abinScience
SKU: HB766207
Price: 196 USD
Availability: InStock

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概要

カタログ番号	HB766207
種反応性	Human
アプリケーション	FCM
宿主種	Mouse
クローン性	Monoclonal
アイソタイプ	IgG1, lambda
クローンID	QBEND-10
テスト済みアプリケーション	FCM
ターゲット	CD34, Hematopoietic progenitor cell antigen CD34
エンドトキシンレベル	Please contact with the lab for this information.
純度	>95% as determined by SDS-PAGE.
精製	Protein A/G purified from cell culture supernatant.
アクセッション番号	P28906
形態	Liquid
保存バッファー	0.01M PBS pH 7.4 データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。
安定性と保存	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
背景	Hematopoietic progenitor cell antigen CD34 is a ~40 kDa protein. Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.
注意事項	For research use only.

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Bioactivity

SEC-HPLC detection for Anti-Human CD34 Antibody (QBEND-10).
Flow Cytometry

Flow-cytometry using anti-human CD34 antibody.CD34 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD34 antibody monoclonal antibody (Catalog # HB766207 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human CD34 antibody.KG-1 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD34 antibody monoclonal antibody (Catalog # HB766207 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.