Anti-Human CD344/FZD4 Recombinant Antibody (5017#), PE (HV376127)

Name: Anti-Human CD344/FZD4 Recombinant Antibody (5017#), PE
Brand: abinScience
SKU: HV376127
Price: 290 USD
Availability: InStock

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SDS

Vista general

Número de catálogo	HV376127
Reactividad de especies	Human
Aplicaciones	FCM
Especie huésped	Mouse
Clonalidad	Monoclonal
Isotipo	IgG1, kappa
ID de clon	5017#
Conjugación	PE
Objetivo	Fz-4, Frizzled-4, CD344, FzE4, FZD4, hFz4
Nivel de endotoxinas	Please contact with the lab for this information.
Productos asociados	Mouse IgG1, kappa Recombinant Isotype Control Antibody (MOPC-21), PE
Número de acceso	Q9ULV1
Forma	Liquid
Buffer de almacenamiento	0.2M Borate buffer pH8.5, 0.02% BSA, 0.05% Proclin 300 Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Estabilidad y almacenamiento	Store at 4°C for 12 months. Protect from light. Do not freeze.
Fondo	Frizzled-4 (CD344/FZD4) is a ~59 kDa protein. Receptor for Wnt proteins. Most frizzled receptors are coupled to the beta-catenin (CTNNB1) canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin (CTNNB1) and activation of Wnt target genes. Plays a critical role in retinal vascularization by acting as a receptor for Wnt proteins and norrin (NDP). In retina, it can be activated by Wnt protein-binding and also by Wnt-independent signaling via binding of norrin (NDP), promoting in both cases beta-catenin (CTNNB1) accumulation and stimulation of LEF/TCF-mediated transcriptional programs. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. 1. Yang, S. et al. (2018) Nature 560, 666-670. PMID: 30135577
Nota	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

Imágenes

Flow CytoMetry

Flow-cytometry using PE anti-human CD344 antibody. HepG-2 cells were stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CD344 monoclonal antibody (Catalog: HV376127, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, and cells analysed on a NovoCyte Flow Cytometer.

Referencias

Datasheet

Anti-Human CD344/FZD4 Recombinant Antibody (5017#), PE.pdf

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