Anti-Human CD344/FZD4 Recombinant Antibody (5017#) (HV376107)

Name: Anti-Human CD344/FZD4 Recombinant Antibody (5017#)
Brand: abinScience
SKU: HV376107
Price: 191 USD
Availability: InStock

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Overview

Catalog No.	HV376107
Species reactivity	Human
Applications	FCM
Host species	Mouse
Isotype	IgG1, kappa
Clone ID	5017#
Clonality	Monoclonal
Target	Fz-4, Frizzled-4, CD344, FzE4, FZD4, hFz4
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	Q9ULV1
Form	Liquid
Storage buffer	0.01M PBS pH 7.4 Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background	Frizzled-4 (CD344/FZD4) is a ~59 kDa protein. Receptor for Wnt proteins. Most frizzled receptors are coupled to the beta-catenin (CTNNB1) canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin (CTNNB1) and activation of Wnt target genes. Plays a critical role in retinal vascularization by acting as a receptor for Wnt proteins and norrin (NDP). In retina, it can be activated by Wnt protein-binding and also by Wnt-independent signaling via binding of norrin (NDP), promoting in both cases beta-catenin (CTNNB1) accumulation and stimulation of LEF/TCF-mediated transcriptional programs. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. 1. Yang, S. et al. (2018) Nature 560, 666-670. PMID: 30135577
Note	For research use only.

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Bioactivity

Detects CD344/FZD4 in indirect ELISAs.
Flow Cytometry

Flow-cytometry using anti-human CD344 antibody.HeLa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD344 antibody monoclonal antibody (Catalog # HV376107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet

Anti-Human CD344/FZD4 Recombinant Antibody (5017#).pdf

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Formula

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Recombinant Proteins

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Anti-Human CD344/FZD4 Recombinant Antibody (5017#) (HV376107)

Overview

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Datasheet

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