Anti-Human ICAM-1/CD54 Recombinant Antibody (SAA0025) (HY321107)

Name: Anti-Human ICAM-1/CD54 Recombinant Antibody (SAA0025)
Brand: abinScience
SKU: HY321107
Price: 196 USD
Availability: InStock

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Изображения
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Даташит
SDS

Обзор

Каталожный номер	HY321107
Реактивность видов	Human
Применения	ELISA, FCM
Хозяинский вид	Human
Клоональность	Monoclonal
Изотип	IgG1, lambda
ID клона	SAA0025
Проверенные приложения	FCM
Цель	ICAM1, Major group rhinovirus receptor, CD54, ICAM-1, Intercellular adhesion molecule 1
Уровень эндотоксинов	Please contact with the lab for this information.
Чистота	>95% as determined by SDS-PAGE.
Очистка	Protein A/G purified from cell culture supernatant.
Номер доступа	P05362
Форма	Liquid
Буфер хранения	0.01M PBS pH 7.4 Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Устойчивость и хранение	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Фон	Intercellular adhesion molecule 1 (CD54/ICAM1) is a ~57 kDa protein. Cell adhesion molecule that functions as a receptor ligand of the signaling receptor ITGAL:ITGB2/LFA-1 (lymphocyte-function associated (LFA) molecule 1) ensuring leukocyte cell-cell adhesion, by providing a calibrated system to namely adjust T-cell killing to the antigen stimulation strength. Also functions as a ligand receptor of the signaling receptor ITGAM:ITGB2/MAC-1 ensuring adhesion between stimulated neutrophils and stimulated endothelial cells. During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation. Promotes cell aggregation in epithelial cells through interaction with MUC1. 1. Dustin, ML. et al. (1986) Journal of immunology (Baltimore, Md. : 1950) 137, 245-54. PMID: 3086451 2. Simmons, D. et al. (1988) Nature 331, 624-7. PMID: 3340213 3. Lacouture, C. et al. (2024) Nature communications 15, 407. PMID: 38195629 4. Diamond, MS. et al. (1990) The Journal of cell biology 111, 3129-39. PMID: 1980124 5. van Buul, JD. et al. (2007) The Journal of cell biology 178, 1279-93. PMID: 17875742 6. Hayashi, T. et al. (2001) Digestion 63 Suppl 1, 87-92. PMID: 11173916
Примечание	For research use only.

Изображения

Flow-cytometry

Flow-cytometry using anti- human CD54 antibody.
Human RPMI-8226 cell line were stained with an irrelevant antibody (Gray Histogram) or an anti- human CD54 antibody monoclonal antibody (Catalog # HY321107 , Red Histogram) at a concentration of 20 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Bioactivity

SEC-HPLC detection for Anti-Human CD54/ICAM1 Antibody (ABS1990).