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Anti-Human CEACAM-8/CD66b Recombinant Antibody (BW250/183) (HB202107)

Anti-Human CEACAM-8/CD66b Recombinant Antibody (BW250/183)
Anti-Human CEACAM-8/CD66b Recombinant Antibody (BW250/183)
Anti-Human CEACAM-8/CD66b Recombinant Antibody (BW250/183)
Anti-Human CEACAM-8/CD66b Recombinant Antibody (BW250/183)
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Aperçu
Numéro de catalogueHB202107
Reactivité des espècesHuman
ApplicationsFCM
Espèce hôteMouse
ClonalitéMonoclonal
IsotypeIgG1, kappa
ID de cloneBW250/183
Cible CD67 antigen, Carcinoembryonic antigen CGM6, Carcinoembryonic antigen-related cell adhesion molecule 8, CGM6, CD66b, Non-specific cross-reacting antigen NCA-95, CEACAM8
Niveau d'endotoxines Please contact with the lab for this information.
Pureté >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Numéro d'accès P31997
Forme Liquid
Tampon de stockage 0.01M PBS pH 7.4

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Contexte

Cell adhesion molecule CEACAM8 (CD66b) is a ~38 kDa protein. Cell surface glycoprotein that plays a role in cell adhesion in a calcium-independent manner. Mediates heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM6. Heterophilic interaction with CEACAM8 occurs in activated neutrophils.

1. Kuroki, M. et al. (2001) Journal of leukocyte biology 70, 543-50. PMID: 11590190
2. Oikawa, S. et al. (1991) The Journal of biological chemistry 266, 7995-8001. PMID: 2022629
3. Yamanaka, T. et al. (1996) Protein expression and purification 7, 438-46. PMID: 8776764
Note For research use only.
Images
  • Anti-Human CEACAM-8/CD66b Recombinant Antibody (BW250/183)

    Bioactivity

    SEC-HPLC detection for Anti-Human CD66b/CEACAM8 Antibody (BW250/183).

  • Anti-Human CEACAM-8/CD66b Recombinant Antibody (BW250/183)

    Flow CytoMetry

    Flow-cytometry using anti-human CD66b antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CD66b monoclonal antibody (Catalog: HB202107) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.

Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Recommandation

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