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Anti-Human CD7 Recombinant Antibody (WT1), FITC (HY302117)

Anti-Human CD7 Recombinant Antibody (WT1), FITC
Anti-Human CD7 Recombinant Antibody (WT1), FITC
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  • 100T
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Vista general
Número de catálogoHY302117
Reactividad de especiesHuman
AplicacionesFCM
Especie huéspedMouse
ClonalidadMonoclonal
IsotipoIgG2a, Lambda
ID de clonWT1
ConjugaciónFITC
Aplicaciones probadas FCM
Objetivo GP40, CD7, T-cell surface antigen Leu-9, T-cell leukemia antigen, TP41, T-cell antigen CD7
Nivel de endotoxinas Please contact with the lab for this information.
Productos asociados Mouse IgG2a Recombinant Isotype Control Antibody (C1.18.4), FITC
Número de acceso P09564
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Estabilidad y almacenamiento Store at 4°C for 12 months. Protect from light. Do not freeze.
Fondo

T-cell antigen CD7 is a ~25 kDa protein. Transmembrane glycoprotein expressed by T-cells and natural killer (NK) cells and their precursors. Plays a costimulatory role in T-cell activation upon binding to its ligand K12/SECTM1. In turn, mediates the production of cytokines such as IL-2. On resting NK-cells, CD7 activation results in a significant induction of interferon-gamma levels.

1. Rabinowich, H. et al. (1994) Journal of immunology (Baltimore, Md. : 1950) 152, 517-26. PMID: 7506726
2. Lyman, SD. et al. (2000) The Journal of biological chemistry 275, 3431-7. PMID: 10652336
3. Carrel, S. et al. (1991) European journal of immunology 21, 1195-200. PMID: 1709867
Nota For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.
Imágenes
  • Anti-Human CD7 Recombinant Antibody (WT1), FITC

    Flow CytoMetry

    Flow-cytometry using FITC anti-human CD7 antibody. Jurkat cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CD7 monoclonal antibody (Catalog: HY302117, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

Referencias
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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