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Anti-Human CD7 Recombinant Antibody (WT1), FITC (HY302117)

Anti-Human CD7 Recombinant Antibody (WT1), FITC
Anti-Human CD7 Recombinant Antibody (WT1), FITC
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Aperçu
Numéro de catalogueHY302117
Reactivité des espècesHuman
ApplicationsFCM
Espèce hôteMouse
ClonalitéMonoclonal
IsotypeIgG2a, Lambda
ID de cloneWT1
ConjugaisonFITC
Applications testées FCM
Cible GP40, CD7, T-cell surface antigen Leu-9, T-cell leukemia antigen, TP41, T-cell antigen CD7
Niveau d'endotoxines Please contact with the lab for this information.
Produits associés Mouse IgG2a Recombinant Isotype Control Antibody (C1.18.4), FITC
Numéro d'accès P09564
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Stabilité et stockage Store at 4°C for 12 months. Protect from light. Do not freeze.
Contexte

T-cell antigen CD7 is a ~25 kDa protein. Transmembrane glycoprotein expressed by T-cells and natural killer (NK) cells and their precursors. Plays a costimulatory role in T-cell activation upon binding to its ligand K12/SECTM1. In turn, mediates the production of cytokines such as IL-2. On resting NK-cells, CD7 activation results in a significant induction of interferon-gamma levels.

1. Rabinowich, H. et al. (1994) Journal of immunology (Baltimore, Md. : 1950) 152, 517-26. PMID: 7506726
2. Lyman, SD. et al. (2000) The Journal of biological chemistry 275, 3431-7. PMID: 10652336
3. Carrel, S. et al. (1991) European journal of immunology 21, 1195-200. PMID: 1709867
Note For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.
Images
  • Anti-Human CD7 Recombinant Antibody (WT1), FITC

    Flow CytoMetry

    Flow-cytometry using FITC anti-human CD7 antibody. Jurkat cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CD7 monoclonal antibody (Catalog: HY302117, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
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Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
Recommandation

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