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Anti-Human CD7 Recombinant Antibody (WT1) (HY302107)

Anti-Human CD7 Recombinant Antibody (WT1)
Anti-Human CD7 Recombinant Antibody (WT1)
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Especificación:
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  • 100ug
Número:
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Vista general
Número de catálogoHY302107
Reactividad de especiesHuman
AplicacionesFCM
Especie huéspedMouse
ClonalidadMonoclonal
IsotipoIgG2a, Lambda
ID de clonWT1
Aplicaciones probadas FCM
Objetivo GP40, CD7, T-cell surface antigen Leu-9, T-cell leukemia antigen, TP41, T-cell antigen CD7
Nivel de endotoxinas Please contact with the lab for this information.
Pureza >95% as determined by SDS-PAGE.
Purificación Protein A/G purified from cell culture supernatant.
Número de acceso P09564
Forma Liquid
Buffer de almacenamiento 0.01M PBS pH 7.4

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Fondo

T-cell antigen CD7 is a ~25 kDa protein. Transmembrane glycoprotein expressed by T-cells and natural killer (NK) cells and their precursors. Plays a costimulatory role in T-cell activation upon binding to its ligand K12/SECTM1. In turn, mediates the production of cytokines such as IL-2. On resting NK-cells, CD7 activation results in a significant induction of interferon-gamma levels.

1. Rabinowich, H. et al. (1994) Journal of immunology (Baltimore, Md. : 1950) 152, 517-26. PMID: 7506726
2. Lyman, SD. et al. (2000) The Journal of biological chemistry 275, 3431-7. PMID: 10652336
3. Carrel, S. et al. (1991) European journal of immunology 21, 1195-200. PMID: 1709867
Nota For research use only.
Imágenes
  • Anti-Human CD7 Recombinant Antibody (WT1)

    Flow Cytometry

    Flow-cytometry using anti-human CD7 antibody. Human Jurkat cell line were stained with an irrelevant antibody (Green Histogram) or an anti-human CD7 antibody monoclonal antibody (Catalog # HY302107 , Red Histogram) at a concentration of 20µg/ml for 30 mins at RT. After washing, bound antibody was detected using a CoraLite488 conjugated goat anti-mouse antibody (Catalog # MF690314) and cells analysed on a NovoCyte Flow Cytometer.

Referencias
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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