Anti-Human CD7 Recombinant Antibody (WT1), FITC (HY302117)

Name: Anti-Human CD7 Recombinant Antibody (WT1), FITC
Brand: abinScience
SKU: HY302117
Price: 196 USD
Availability: InStock

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Overview

Catalog No.	HY302117
Species reactivity	Human
Applications	FCM
Host species	Mouse
Isotype	IgG2a, Lambda
Clone ID	WT1
Conjugation	FITC
Clonality	Monoclonal
Target	GP40, CD7, T-cell surface antigen Leu-9, T-cell leukemia antigen, TP41, T-cell antigen CD7
Endotoxin level	Please contact with the lab for this information.
Accession	P09564
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Store at 4°C for 12 months. Protect from light. Do not freeze.
Background	T-cell antigen CD7 is a ~25 kDa protein. Transmembrane glycoprotein expressed by T-cells and natural killer (NK) cells and their precursors. Plays a costimulatory role in T-cell activation upon binding to its ligand K12/SECTM1. In turn, mediates the production of cytokines such as IL-2. On resting NK-cells, CD7 activation results in a significant induction of interferon-gamma levels. 1. Rabinowich, H. et al. (1994) Journal of immunology (Baltimore, Md. : 1950) 152, 517-26. PMID: 7506726 2. Lyman, SD. et al. (2000) The Journal of biological chemistry 275, 3431-7. PMID: 10652336 3. Carrel, S. et al. (1991) European journal of immunology 21, 1195-200. PMID: 1709867
Note	For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.

Images

Flow CytoMetry

Flow-cytometry using FITC anti-human CD7 antibody. Jurkat cells were stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CD7 monoclonal antibody (Catalog: HY302117, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet