Anti-Human CD7 Recombinant Antibody (WT1) (HY302107)

Name: Anti-Human CD7 Recombinant Antibody (WT1)
Brand: abinScience
SKU: HY302107
Price: 196 USD
Availability: InStock

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Numéro de catalogue	HY302107
Reactivité des espèces	Human
Applications	FCM
Espèce hôte	Mouse
Clonalité	Monoclonal
Isotype	IgG2a, Lambda
ID de clone	WT1
Applications testées	FCM
Cible	GP40, CD7, T-cell surface antigen Leu-9, T-cell leukemia antigen, TP41, T-cell antigen CD7
Niveau d'endotoxines	Please contact with the lab for this information.
Pureté	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Numéro d'accès	P09564
Forme	Liquid
Tampon de stockage	0.01M PBS pH 7.4 Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Contexte	T-cell antigen CD7 is a ~25 kDa protein. Transmembrane glycoprotein expressed by T-cells and natural killer (NK) cells and their precursors. Plays a costimulatory role in T-cell activation upon binding to its ligand K12/SECTM1. In turn, mediates the production of cytokines such as IL-2. On resting NK-cells, CD7 activation results in a significant induction of interferon-gamma levels. 1. Rabinowich, H. et al. (1994) Journal of immunology (Baltimore, Md. : 1950) 152, 517-26. PMID: 7506726 2. Lyman, SD. et al. (2000) The Journal of biological chemistry 275, 3431-7. PMID: 10652336 3. Carrel, S. et al. (1991) European journal of immunology 21, 1195-200. PMID: 1709867
Note	For research use only.

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Flow Cytometry

Flow-cytometry using anti-human CD7 antibody. Human Jurkat cell line were stained with an irrelevant antibody (Green Histogram) or an anti-human CD7 antibody monoclonal antibody (Catalog # HY302107 , Red Histogram) at a concentration of 20µg/ml for 30 mins at RT. After washing, bound antibody was detected using a CoraLite488 conjugated goat anti-mouse antibody (Catalog # MF690314) and cells analysed on a NovoCyte Flow Cytometer.

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