Anti-Human Transferrin R/TfR Reference Recombinant Antibody (SAA1396) (HY036207)

Name: Anti-Human Transferrin R/TfR Reference Recombinant Antibody (SAA1396)
Brand: abinScience
SKU: HY036207
Price: 191 USD
Availability: InStock

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Каталожный номер	HY036207
Реактивность видов	Human
Применения	FCM
Хозяинский вид	Mouse
Клоональность	Monoclonal
Изотип	IgG2a, kappa
ID клона	SAA1396
Проверенные приложения	FCM
Цель	CD71, T9, TFRC, TR, TfR, TfR1, Transferrin receptor 1, Transferrin receptor protein 1, Transferrin receptor protein 1, serum form, Trfr, p90, sTfR
Уровень эндотоксинов	< 10 EU/mg
Чистота	>95% as determined by SDS-PAGE.
Очистка	Protein A/G purified from cell culture supernatant.
Номер доступа	P02786
Форма	Liquid
Буфер хранения	0.01M PBS pH 7.4. Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Устойчивость и хранение	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Фон	Transferrin receptor protein 1 (CD71/TFRC) is a ~84 kDa protein. Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system. A second ligand, the hereditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. 1. Senyilmaz, D. et al. (2015) Nature 525, 124-8. PMID: 26214738 2. Jabara, HH. et al. (2016) Nature genetics 48, 74-8. PMID: 26642240
Примечание	For research use only.

Изображения

Flow-cytometry

Flow-cytometry using anti-human CD71 antibody.CD71 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD71 antibody monoclonal antibody (Catalog # HY036207 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry

Flow-cytometry using anti-human CD71 antibody.U937 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD71 antibody monoclonal antibody (Catalog # HY036207 ,Green Histogram) at a concentration of 5 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using anti-human CD71 antibody. U937 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD71 monoclonal antibody (Catalog: HY036207, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.