Anti-Human CD99 Recombinant Antibody (12E7#) (HB677107)

Name: Anti-Human CD99 Recombinant Antibody (12E7#)
Brand: abinScience
SKU: HB677107
Price: 196 USD
Availability: InStock

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Número de catálogo	HB677107
Reactividad de especies	Human
Aplicaciones	FCM
Especie huésped	Mouse
Clonalidad	Monoclonal
Isotipo	IgG1, kappa
ID de clon	12E7#
Objetivo	MIC2Y, E2 antigen, MIC2X, 12E7, Protein MIC2, CD99 antigen, MIC2, T-cell surface glycoprotein E2, CD99
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>95% as determined by SDS-PAGE.
Purificación	Protein A/G purified from cell culture supernatant.
Número de acceso	P14209
Forma	Liquid
Buffer de almacenamiento	0.01M PBS pH 7.4 Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Fondo	CD99 antigen is a ~18 kDa protein. Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Involved in T-cell adhesion processes.
Nota	For research use only.

Imágenes

Bioactivity

Detects CD99 in indirect ELISAs.
Flow Cytometry

Flow-cytometry using anti-human CD99 antibody.CD99 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD99 antibody monoclonal antibody (Catalog # HB677107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human CD99 antibody.Human peripheral blood monocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD99 antibody monoclonal antibody (Catalog # HB677107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human CD99 antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD99 antibody monoclonal antibody (Catalog # HB677107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

Referencias

Datasheet

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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