Anti-Human CD99 Recombinant Antibody (12E7#) (HB677107)

Name: Anti-Human CD99 Recombinant Antibody (12E7#)
Brand: abinScience
SKU: HB677107
Price: 196 USD
Availability: InStock

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概要

カタログ番号	HB677107
種反応性	Human
アプリケーション	FCM
宿主種	Mouse
クローン性	Monoclonal
アイソタイプ	IgG1, kappa
クローンID	12E7#
ターゲット	MIC2Y, E2 antigen, MIC2X, 12E7, Protein MIC2, CD99 antigen, MIC2, T-cell surface glycoprotein E2, CD99
エンドトキシンレベル	Please contact with the lab for this information.
純度	>95% as determined by SDS-PAGE.
精製	Protein A/G purified from cell culture supernatant.
アクセッション番号	P14209
形態	Liquid
保存バッファー	0.01M PBS pH 7.4 データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。
安定性と保存	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
背景	CD99 antigen is a ~18 kDa protein. Involved in T-cell adhesion processes and in spontaneous rosette formation with erythrocytes. Plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Acts at the same site as, but independently of, PECAM1. Involved in T-cell adhesion processes.
注意事項	For research use only.

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Bioactivity

Detects CD99 in indirect ELISAs.
Flow Cytometry

Flow-cytometry using anti-human CD99 antibody.CD99 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD99 antibody monoclonal antibody (Catalog # HB677107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human CD99 antibody.Human peripheral blood monocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD99 antibody monoclonal antibody (Catalog # HB677107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human CD99 antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD99 antibody monoclonal antibody (Catalog # HB677107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

参考文献

データシート

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概要