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Anti-Human CDCA7 Polyclonal Antibody (HP761014)

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Vista general
Número de catálogoHP761014
Descripción
Anti-Human CDCA7 Polyclonal Antibody (HP761014) is a rabbit polyclonal antibody detecting CDCA7 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Reactividad de especiesHuman, Mouse, Rat
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human CDCA7 (Thr246-Ala371).
Objetivo CDCA7, JPO1, Cell division cycle-associated protein 7, Protein JPO1
Purificación Purified by antigen affinity column.
Número de acceso Q9BWT1
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Cell division cycle-associated protein 7 (CDCA7) is a ~42 kDa protein. Participates in MYC-mediated cell transformation and apoptosis; induces anchorage-independent growth and clonogenicity in lymphoblastoid cells. Insufficient to induce tumorigenicity when overexpressed but contributes to MYC-mediated tumorigenesis. Also functions as a critical cofactor for the chromatin remodeler HELLS, facilitating its recruitment to specific genomic regions to maintain DNA methylation patterns and heterochromatin integrity. Recognizes hemimethylated CpG within nucleosomes where it recruits HELLS to remodel chromatin and facilitate access of de novo DNA methyltransferases to heterochromatic regions, enabling proper establishment of DNA methylation patterns. May play a role as transcriptional regulator.

1. Prescott, JE. et al. (2001) The Journal of biological chemistry 276, 48276-84. PMID: 11598121
2. Osthus, RC. et al. (2005) Cancer research 65, 5620-7. PMID: 15994934
3. Gill, RM. et al. (2013) Molecular and cellular biology 33, 498-513. PMID: 23166294
4. Unoki, M. et al. (2019) The Journal of clinical investigation 129, 78-92. PMID: 30307408
5. Wassing, IE. et al. (2024) Science advances 10, eadp5753. PMID: 39178260
6. Goto, Y. et al. (2006) Biochimica et biophysica acta 1759, 60-8. PMID: 16580749
Nota For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human CDCA7 Polyclonal Antibody

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