Human CDCA7 Recombinant Protein (N-His) (HP761012)

Name: Human CDCA7 Recombinant Protein (N-His)
Brand: abinScience
SKU: HP761012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HP761012
Description	Recombinant Human CDCA7 Protein, N-His (HP761012) expressed in E. coli, spanning Thr246-Ala371. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q9BWT1
Protein length	Thr246-Ala371
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	16.78 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	CDCA7, JPO1, Cell division cycle-associated protein 7, Protein JPO1
Background	Cell division cycle-associated protein 7 (CDCA7) is a ~42 kDa protein. Participates in MYC-mediated cell transformation and apoptosis; induces anchorage-independent growth and clonogenicity in lymphoblastoid cells. Insufficient to induce tumorigenicity when overexpressed but contributes to MYC-mediated tumorigenesis. Also functions as a critical cofactor for the chromatin remodeler HELLS, facilitating its recruitment to specific genomic regions to maintain DNA methylation patterns and heterochromatin integrity. Recognizes hemimethylated CpG within nucleosomes where it recruits HELLS to remodel chromatin and facilitate access of de novo DNA methyltransferases to heterochromatic regions, enabling proper establishment of DNA methylation patterns. May play a role as transcriptional regulator. 1. Prescott, JE. et al. (2001) The Journal of biological chemistry 276, 48276-84. PMID: 11598121 2. Osthus, RC. et al. (2005) Cancer research 65, 5620-7. PMID: 15994934 3. Gill, RM. et al. (2013) Molecular and cellular biology 33, 498-513. PMID: 23166294 4. Unoki, M. et al. (2019) The Journal of clinical investigation 129, 78-92. PMID: 30307408 5. Wassing, IE. et al. (2024) Science advances 10, eadp5753. PMID: 39178260 6. Goto, Y. et al. (2006) Biochimica et biophysica acta 1759, 60-8. PMID: 16580749
Note	For research use only.

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SDS-PAGE for Recombinant Human CDCA7 Protein, N-His

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Datasheet

Human CDCA7 Recombinant Protein (N-His).pdf

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Human CDCA7 Recombinant Protein (N-His) (HP761012)

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