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Anti-Human CDT1 Polyclonal Antibody (HD509014)

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Anti-Human CDT1 Polyclonal Antibody
Anti-Human CDT1 Polyclonal Antibody
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  • 100ug
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Vista general
Número de catálogoHD509014
Descripción
Anti-Human CDT1 Polyclonal Antibody (HD509014) is a rabbit polyclonal antibody detecting CDT1. Suitable for Human.
Reactividad de especiesHuman
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human CDT1 (Thr112-Ser386).
Objetivo CDT1, DNA replication factor Cdt1, DUP, Double parked homolog
Nivel de endotoxinas Please contact with the lab for this information.
Purificación Purified by antigen affinity column.
Número de acceso Q9H211
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Fondo

DNA replication factor Cdt1 is a ~60 kDa protein. Required for both DNA replication and mitosis. DNA replication licensing factor, required for pre-replication complex assembly. Cooperates with CDC6 and the origin recognition complex (ORC) during G1 phase of the cell cycle to promote the loading of the mini-chromosome maintenance (MCM) complex onto DNA to generate pre-replication complexes (pre-RC). Required also for mitosis by promoting stable kinetochore-microtubule attachments. Potential oncogene.

1. Wohlschlegel, JA. et al. (2000) Science (New York, N.Y.) 290, 2309-12. PMID: 11125146
2. Sugimoto, N. et al. (2004) The Journal of biological chemistry 279, 19691-7. PMID: 14993212
3. Miotto, B. et al. (2011) Molecular cell 44, 62-71. PMID: 21856198
4. Varma, D. et al. (2012) Nature cell biology 14, 593-603. PMID: 22581055
5. Franz, A. et al. (2016) Nature communications 7, 10612. PMID: 26842564
6. Cook, JG. et al. (2004) The Journal of biological chemistry 279, 9625-33. PMID: 14672932
Nota For research use only
Imágenes
  • Anti-Human CDT1 Polyclonal Antibody

    Western blot

    Lane 1: HCT116 cell lysate
    Lane 2: Jurkat cell lysate
    Lane 3: K562 cell lysate
    Lane 4: MCF-7 cell lysate
    Lane 5: Hela cell lysate

Referencias
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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