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Anti-Human CFH Recombinant Antibody (SAA0527) (HY505013)

Anti-Human CFH Recombinant Antibody (SAA0527)
Anti-Human CFH Recombinant Antibody (SAA0527)
Anti-Human CFH Recombinant Antibody (SAA0527)
Anti-Human CFH Recombinant Antibody (SAA0527)
Anti-Human CFH Recombinant Antibody (SAA0527)
Anti-Human CFH Recombinant Antibody (SAA0527)
Anti-Human CFH Recombinant Antibody (SAA0527)
Anti-Human CFH Recombinant Antibody (SAA0527)
Anti-Human CFH Recombinant Antibody (SAA0527)
Anti-Human CFH Recombinant Antibody (SAA0527)
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Aperçu
Numéro de catalogueHY505013
Reactivité des espècesHuman
ApplicationsELISA, FCM, WB
Espèce hôteMouse
ClonalitéMonoclonal
IsotypeIgG2a, kappa
ID de cloneSAA0527
Cible Complement factor H, CFH, H factor 1, HF1, HF, HF2
Niveau d'endotoxines Please contact with the lab for this information.
Pureté >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Numéro d'accès P08603
RRID Anti-Human CFH Antibody (SAA0527) (abinScience Cat# HY505013, RRID:AB_3726045)
Forme Liquid
Tampon de stockage 0.01M PBS pH 7.4

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit
Application Dilution
ELISA 1:2000-1:20000
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Note For research use only.
Isotype Control Mouse IgG2a (Fc Silence) Recombinant Isotype Control (MOPC 173) (MF533023)
Images
  • Anti-Human CFH Recombinant Antibody (SAA0527)

    Flow Cytometry

    Flow-cytometry using anti-human CFH antibody.HepG2 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CFH antibody monoclonal antibody (Catalog # HY505013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

  • Anti-Human CFH Recombinant Antibody (SAA0527)

    SDS-PAGE

    SDS-PAGE for Anti-Human CFH Antibody (SAA0527).

  • Anti-Human CFH Recombinant Antibody (SAA0527)

    Flow CytoMetry

    Flow-cytometry using PE anti-human CFH antibody. HepG-2 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an PE anti-human CFH monoclonal antibody (Catalog: HY505013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

  • Anti-Human CFH Recombinant Antibody (SAA0527)

    Flow CytoMetry

    Flow-cytometry using FITC anti-human CFH antibody. HepG-2 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CFH monoclonal antibody (Catalog: HY505013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

  • Anti-Human CFH Recombinant Antibody (SAA0527)

    Flow CytoMetry

    Flow-cytometry using APC anti-human CFH antibody. HepG-2 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an APC anti-human CFH monoclonal antibody (Catalog: HY505013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Recommandation

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