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Каталожный номерHP690014
Описание
Anti-Human CHAC1 Polyclonal Antibody (HP690014) is a rabbit polyclonal antibody detecting CHAC1/Botch in ELISA, IHC, WB. Suitable for Human, Mouse, Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Реактивность видовHuman, Mouse, Rat
ПримененияELISA, IHC, WB
Хозяинский видRabbit
КлоональностьPolyclonal
ИзотипIgG
Иммуноген E. coli - derived recombinant Human CHAC1 (Gln31-Val222).
Цель Glutathione-specific gamma-glutamylcyclotransferase 1, Gamma-GCG 1, Cation transport regulator-like protein 1, CHAC1, Botch, BOTCH, Blocks Notch protein
Очистка Purified by antigen affinity column.
Номер доступа Q9BUX1
Форма Liquid
Буфер хранения 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

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Информация об использовании продукта
Применение Разбавление
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Фон

Glutathione-specific gamma-glutamylcyclotransferase 1 (CHAC1/Botch) is a ~24 kDa protein. Catalyzes the cleavage of glutathione into 5-oxo-L-proline and a Cys-Gly dipeptide. Acts specifically on glutathione, but not on other gamma-glutamyl peptides. Glutathione depletion is an important factor for apoptosis initiation and execution. Acts as a pro-apoptotic component of the unfolded protein response pathway by mediating the pro-apoptotic effects of the ATF4-ATF3-DDIT3/CHOP cascade. Negative regulator of Notch signaling pathway involved in embryonic neurogenesis: acts by inhibiting Notch cleavage by furin, maintaining Notch in an immature inactive form, thereby promoting neurogenesis in embryos.

1. Kaur, A. et al. (2017) The Journal of biological chemistry 292, 638-651. PMID: 27913623
2. Mungrue, IN. et al. (2009) Journal of immunology (Baltimore, Md. : 1950) 182, 466-76. PMID: 19109178
3. Chi, Z. et al. (2012) Developmental cell 22, 707-20. PMID: 22445366
Примечание For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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