Human CHAC1 Recombinant Protein (N-His) (HP690012)

Name: Human CHAC1 Recombinant Protein (N-His)
Brand: abinScience
SKU: HP690012
Price: 211 USD
Availability: InStock

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Overview

Catalog No.	HP690012
Description	Recombinant Human CHAC1 Protein, N-His (HP690012) expressed in E. coli, spanning Gln31-Val222. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q9BUX1
Protein length	Gln31-Val222
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	23.58 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Glutathione-specific gamma-glutamylcyclotransferase 1, Gamma-GCG 1, Cation transport regulator-like protein 1, CHAC1, Botch, BOTCH, Blocks Notch protein
Background	Glutathione-specific gamma-glutamylcyclotransferase 1 (CHAC1/Botch) is a ~24 kDa protein. Catalyzes the cleavage of glutathione into 5-oxo-L-proline and a Cys-Gly dipeptide. Acts specifically on glutathione, but not on other gamma-glutamyl peptides. Glutathione depletion is an important factor for apoptosis initiation and execution. Acts as a pro-apoptotic component of the unfolded protein response pathway by mediating the pro-apoptotic effects of the ATF4-ATF3-DDIT3/CHOP cascade. Negative regulator of Notch signaling pathway involved in embryonic neurogenesis: acts by inhibiting Notch cleavage by furin, maintaining Notch in an immature inactive form, thereby promoting neurogenesis in embryos. 1. Kaur, A. et al. (2017) The Journal of biological chemistry 292, 638-651. PMID: 27913623 2. Mungrue, IN. et al. (2009) Journal of immunology (Baltimore, Md. : 1950) 182, 466-76. PMID: 19109178 3. Chi, Z. et al. (2012) Developmental cell 22, 707-20. PMID: 22445366
Note	For research use only.

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SDS-PAGE for Recombinant Human CHAC1 Protein, N-His

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Datasheet

Human CHAC1 Recombinant Protein (N-His).pdf

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Human CHAC1 Recombinant Protein (N-His) (HP690012)

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