Anti-Human CUL1 Polyclonal Antibody (HC489014)

Name: Anti-Human CUL1 Polyclonal Antibody
Brand: abinScience
SKU: HC489014
Price: 96 USD
Availability: InStock

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Número de catálogo

HC489014

Descripción

Anti-Human CUL1 Polyclonal Antibody (HC489014) is a rabbit polyclonal antibody detecting CUL1 in ELISA, IHC, WB. Suitable for Mus musculus and Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Reactividad de especies

Human, Mouse

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant Human CUL1 (Met1-Glu250).

Objetivo

CUL1

Purificación

Purified by antigen affinity column.

Número de acceso

Q13616

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Nombres alternativos

Cullin-1, CUL-1, CUL1

Fondo

Cullin-1 (CUL1) is a ~89 kDa protein. Core component of multiple cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. SCF complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins. In the SCF complex, serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits. May contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and exchange of the substrate recognition component is mediated by TIP120A/CAND1.

1. Gao, D. et al. (2011) Molecular cell 44, 290-303. PMID: 22017875

2. Duan, S. et al. (2011) Molecular cell 44, 317-24. PMID: 22017877

3. Scott, DC. et al. (2016) Cell 166, 1198-1214.e24. PMID: 27565346

4. Liwocha, J. et al. (2024) Nature structural & molecular biology 31, 378-389. PMID: 38326650

5. Min, KW. et al. (2003) The Journal of biological chemistry 278, 15905-10. PMID: 12609982

6. Pagan, JK. et al. (2015) Nature cell biology 17, 31-43. PMID: 25503564

7. Man, X. et al. (2015) European journal of cell biology 94, 162-72. PMID: 25704143

8. Hopf, LVM. et al. (2022) Nature structural & molecular biology 29, 854-862. PMID: 35982156

10. Muhar, MF. et al. (2025) Nature 638, 519-527. PMID: 39880951

Nota

For research use only.

Imágenes

Western blot

Western blot analysis was performed using anti-CUL1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Mouse brain lysate
Lane 2: Rat brain lysate

Referencias

Datasheet

Anti-Human CUL1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

Kits de Ensayo

Anti-Human CUL1 Polyclonal Antibody (HC489014)

Vista general

Imágenes

Referencias

Datasheet

SDS