Human CUL1 Recombinant Protein (N-His) (HC489012)

Name: Human CUL1 Recombinant Protein (N-His)
Brand: abinScience
SKU: HC489012
Price: 211 USD
Availability: InStock

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Catalog No.	HC489012
Description	Recombinant Human CUL1 Protein, N-His (HC489012) expressed in E. coli, spanning Met1-Glu250. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q13616
Protein length	Met1-Glu250
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	31.11 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Cullin-1, CUL-1, CUL1
Background	Cullin-1 (CUL1) is a ~89 kDa protein. Core component of multiple cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. SCF complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins. In the SCF complex, serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits. May contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and exchange of the substrate recognition component is mediated by TIP120A/CAND1. 1. Gao, D. et al. (2011) Molecular cell 44, 290-303. PMID: 22017875 2. Duan, S. et al. (2011) Molecular cell 44, 317-24. PMID: 22017877 3. Scott, DC. et al. (2016) Cell 166, 1198-1214.e24. PMID: 27565346 4. Liwocha, J. et al. (2024) Nature structural & molecular biology 31, 378-389. PMID: 38326650 5. Min, KW. et al. (2003) The Journal of biological chemistry 278, 15905-10. PMID: 12609982 6. Pagan, JK. et al. (2015) Nature cell biology 17, 31-43. PMID: 25503564 7. Man, X. et al. (2015) European journal of cell biology 94, 162-72. PMID: 25704143 8. Hopf, LVM. et al. (2022) Nature structural & molecular biology 29, 854-862. PMID: 35982156 10. Muhar, MF. et al. (2025) Nature 638, 519-527. PMID: 39880951
Note	For research use only.

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SDS-PAGE for Recombinant Human CUL1 Protein, N-His

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Datasheet

Human CUL1 Recombinant Protein (N-His).pdf

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Human CUL1 Recombinant Protein (N-His) (HC489012)

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