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| カタログ番号 | HC489014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-Human CUL1 Polyclonal Antibody (HC489014) is a rabbit polyclonal antibody detecting CUL1 in ELISA, IHC, WB. Suitable for Mus musculus and Human.
Highlights
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| 種反応性 | Human, Mouse | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human CUL1 (Met1-Glu250). | ||||||||
| ターゲット | CUL1 | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | Q13616 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 別名 | Cullin-1, CUL-1, CUL1 | ||||||||
| 背景 | Cullin-1 (CUL1) is a ~89 kDa protein. Core component of multiple cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. SCF complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins. In the SCF complex, serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits. May contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and exchange of the substrate recognition component is mediated by TIP120A/CAND1. 1. Gao, D. et al. (2011) Molecular cell 44, 290-303. PMID: 22017875 2. Duan, S. et al. (2011) Molecular cell 44, 317-24. PMID: 22017877 3. Scott, DC. et al. (2016) Cell 166, 1198-1214.e24. PMID: 27565346 4. Liwocha, J. et al. (2024) Nature structural & molecular biology 31, 378-389. PMID: 38326650 5. Min, KW. et al. (2003) The Journal of biological chemistry 278, 15905-10. PMID: 12609982 6. Pagan, JK. et al. (2015) Nature cell biology 17, 31-43. PMID: 25503564 7. Man, X. et al. (2015) European journal of cell biology 94, 162-72. PMID: 25704143 8. Hopf, LVM. et al. (2022) Nature structural & molecular biology 29, 854-862. PMID: 35982156 10. Muhar, MF. et al. (2025) Nature 638, 519-527. PMID: 39880951 | ||||||||
| 注意事項 | For research use only. |
Western blot analysis was performed using anti-CUL1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Mouse brain lysate
Lane 2: Rat brain lysate
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